精子在经受冷冻和解冻时会经历负面变化,包括降低的运动性,活力和顶体反应。在这里,研究了不同浓度的大豆卵磷脂纳米颗粒对冷冻保存的荷斯坦公牛精液的影响。收集精液,冷冻保存并用于稀释后的精子动力学参数分析,用0.5%大豆卵磷脂(E1)平衡和解冻,控制扩展器,和0.75%(E2),0.5%(E3),0.25%(E4)和0.125%(E5)的卵磷脂纳米颗粒。结果表明,稀释后,卵磷脂纳米颗粒在E3、E4和E5的渐进运动(PM)高于E2(p<0.05)。平衡后,与E1、E2和E3值相比,PM,活力,形态正常,E5处的膜完整性和完整顶体值始终更大(p<0.05)。比较E2和E3与E4和E5处的完整顶体和膜完整性的百分比,观察到显著降低(p<.05)。解冻后,尽管与E1、E3和E4相比,它们的平均PM值相似(p>.05),但在E2和E5处PM的百分比改善。精子的活力和进展参数(DAP,DCL,DSL,VAP,VCL,E5的VSL和STR)高于E1、E2、E3和E4。总之,来自荷斯坦公牛的冷冻保存的精子在平衡后和用0.125%卵磷脂纳米颗粒解冻后都表现出出色的特性,他们对高剂量很敏感。
Spermatozoa can experience negative changes when subjected to freezing and thawing, including lowered motility, viability and acrosome response. Herein, the effects of different concentrations of soybean lecithin nanoparticles on cryopreserved Holstein bull semen were examined. Semen was collected, cryopreserved and utilized for sperm kinetic parameter analysis following dilution, equilibration and thawing with 0.5% soybean lecithin (E1), the control extender, and 0.75% (E2), 0.5% (E3), 0.25% (E4) and 0.125% (E5) of lecithin nanoparticles. Results revealed that following dilution, the progressive motility (PM) at E3, E4 and E5 of lecithin nanoparticles was higher (p < .05) than it was for E2. After equilibration, compared to the E1, E2, and E3 values, the PM, vitality, normal morphology, membrane integrity and intact acrosome values at the E5 were consistently greater (p < .05). Comparing the percentages of intact acrosome and membrane integrity at E2 and E3 to E4 and E5, a substantial decrease (p < .05) was seen. Following thawing, the percentage of PM improved at E2 and E5, even though their mean PM values were similar (p > .05) compared to E1, E3 and E4. Vigour and progression parameters of sperm (DAP, DCL, DSL, VAP, VCL, VSL and STR) at E5 were higher (p < .05) than those at E1, E2, E3 and E4. In conclusion, the cryopreserved sperm from Holstein bulls revealed outstanding properties both after equilibration and after thawing with 0.125% lecithin nanoparticles, and they were sensitive to high dosages.