菌株SYSUD00308T,一种短杆状细菌,是从古尔班通古特沙漠收集的沙质土壤中分离出来的,新疆,公关中国。菌株SYSUD00308T为革兰氏染色阴性,有氧,粉红色,不活动,过氧化氢酶和氧化酶阳性。该菌株在4-37℃生长,pH5.0-8.0和0-1.5%(w/v)NaCl。16SrRNA基因测序分析表明,菌株SYSUD00308T属于鲁菲杆菌属,与鲁菲杆菌属MDT1-10-3T表现出最高的序列相似性(97.4%)。总结特征3、4和iso-C15:0是主要的脂肪酸,甲基萘醌7(MK-7)是唯一的呼吸甲基萘醌。极性脂质谱包括磷脂酰乙醇胺,一种身份不明的糖脂,一种身份不明的磷脂,两种身份不明的氨基磷脂,和两种身份不明的脂质.菌株SYSUD00308T的基因组大小和DNAGC含量分别为5,176,683bp和54.8%,分别。SYSUD00308T与Rufibacter属成员之间的平均核苷酸同一性(ANI)和数字DNA-DNA杂交(dDDH)值分别为77.7-81.8%和20.4-23.4%,低于相应的阈值(ANI:95-96%;dDDH:70%)的原核物种定义。根据基因型,表型和系统发育特征,菌株SYSUD00308T代表鲁菲杆菌属的新物种。我们提出了这个名字,鲁菲杆菌属精神病人。11月。,以SYSUD00308T(=CGMCC1.18621T=KCTC82275T=MCCC1K04970T)为类型菌株。
Strain SYSU D00308T, a short-rod-shaped bacterium, was isolated from a sandy soil collected from the Gurbantunggut Desert, Xinjiang, PR China. Strain SYSU D00308T was Gram-stain-negative, aerobic, pink-pigmented, non-motile, catalase- and oxidase-positive. The strain grew at 4-37 ℃, pH 5.0-8.0 and 0-1.5% (w/v) NaCl. 16S rRNA gene sequencing analyses demonstrated that strain SYSU D00308T belonged to the genus Rufibacter and exhibited the highest sequence similarity (97.4%) to Rufibacter glacialis MDT1-10-3T. Summed features 3, 4, and iso-C15:0 were the major fatty acids, and menaquinone 7 (MK-7) was the sole respiratory menaquinone. The polar lipid profiles comprised phosphatidylethanolamine, an unidentified glycolipid, an unidentified phospholipid, two unidentified aminophospholipids, and two unidentified lipids. The genome size and DNA G + C content of strain SYSU D00308T were 5,176,683 bp and 54.8%, respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between SYSU D00308T and members of the genus Rufibacter were 77.7-81.8% and 20.4-23.4% respectively, which were less than the corresponding thresholds (ANI: 95-96%; dDDH: 70%) for prokaryotic species definition. According to the genotypic, phenotypic and phylogenetic characteristics, strain SYSU D00308T represents a novel species of the genus Rufibacter. We propose the name, Rufibacter psychrotolerans sp. nov., with SYSU D00308T (= CGMCC 1.18621T = KCTC 82275T = MCCC 1K04970T) as the type strain.