基于同步加速器的软X射线断层扫描(SXT),提供成像物体的三维形态和线性吸收系数(LAC)的定量分布,在许多领域中广泛用于获取超结构图像,特别是在细胞成像中。结合离线荧光显微镜(FM)以鉴定细胞器的类型和细胞的状态。然而,变形和位移通常发生在传递和加载过程中,这降低了双模态图像配准的精度。在本文中,我们在网上报道FM,在国家同步加速器辐射实验室(NSRL)的SXT站(BL07W),这避免了变形和位移。因此,研究人员可以轻松找到样本并获取有用的数据,而无需繁琐的后处理。将SXT与在线FM相结合,我们实现了凋亡细胞的鉴定和高分辨率成像.实验表明,凋亡细胞的细胞核的LAC大于正常细胞的LAC,这可以用凋亡细胞的核固缩来解释。
Synchrotron-based soft X-ray tomography (SXT), providing three-dimensional morphology and quantitative distribution of linear absorption coefficient (LAC) of the imaged objects, is widely used in many fields to obtain ultra-structure images, especially in cellular imaging. Off-line fluorescence microscopies (FMs) are combined to identify the type of organelles and status of cells. However, deformation and displacement usually occur during the transfer and loading process, which decreases the precision of two-modal images\' registration. In this paper, we report on an on-line FM, at the SXT station (BL07W) of the National Synchrotron Radiation Laboratory (NSRL), which avoids deformation and displacement. Therefore, researchers can easily find the sample and take the useful data without tedious post-processing. Combining SXT with on-line FM, we achieved the identification and high-resolution imaging of an apoptotic cell. The experiments revealed that the LAC of the nucleus of the apoptotic cell was larger than that of a normal cell, which could be explained by nucleus pyknosis of the apoptotic cell.