目的:啮齿动物从其物种中获取食物信息,并显示出对物种消耗食物的偏好。这种对他人食物信息的社会学习促进了一个物种的生存,它被引入作为社会传播的食物偏好(STFP)任务。基底前脑的胆碱能系统在STFP的获取中起作用。大麻二酚(CBD),最丰富的植物大麻素之一,通过多种作用机制发挥其对认知缺陷的治疗潜力,包括它与胆碱能系统的相互作用。我们假设CBD和STFP之间存在正相关关系,因为乙酰胆碱(ACh)参与STFP,CBD会增加基底前脑的ACh水平。
方法:训练雄性C57BL/6J小鼠以获得STFP任务。我们通过施用CBD(20mg/kg,i.p.)在STFP社会培训之前。胆碱能系统参与CBD对STFP的作用通过大脑乙酰胆碱酯酶(AChE)的敲低来检查,应用非选择性毒蕈碱拮抗剂SCO(3mg/kg,i.p.)在CBD治疗之前,并测量CBD治疗小鼠的基底前脑ACh水平。
结果:我们首先表明CBD增强了STFP记忆。大脑AChE的击倒也增强了STFP记忆,这模仿了CBD对STFP的影响。SCO阻断了CBD对STFP的记忆增强作用。我们最重要的发现是,CBD处理的小鼠的基底前脑ACh水平,但不是他们的控制对手,与小鼠的STFP记忆能力呈正相关。
结论:本研究表明CBD增强小鼠的STFP记忆。具体来说,那些通过增加毒蕈碱介导的ACh信号对CBD作出反应的人在其STFP记忆中表现更好。
OBJECTIVE: Rodents acquire food information from their conspecifics and display a preference for the conspecifics\' consumed food. This social learning of food information from others promotes the survival of a species, and it is introduced as the socially transmitted food preference (STFP) task. The cholinergic system in the basal forebrain plays a role in the acquisition of STFP. Cannabidiol (CBD), one of the most abundant phytocannabinoids, exerts its therapeutic potential for cognitive deficits through versatile mechanisms of action, including its interaction with the cholinergic system. We hypothesize a positive relationship between CBD and STFP because acetylcholine (ACh) is involved in STFP, and CBD increases the ACh levels in the basal forebrain.
METHODS: Male C57BL/6J mice were trained to acquire the STFP task. We examined whether CBD affects STFP memory by administering CBD (20 mg/kg, i.p.) before the STFP social training. The involvement of cholinergic system in CBD\'s effect on STFP was examined by knockdown of brain acetylcholinesterase (AChE), applying a nonselective muscarinic antagonist SCO (3 mg/kg, i.p.) before CBD treatment, and measuring the basal forebrain ACh levels in the CBD-treated mice.
RESULTS: We first showed that CBD enhanced STFP memory. Knockdown of brain AChE also enhanced STFP memory, which mimicked CBD\'s effect on STFP. SCO blocked CBD\'s memory-enhancing effect on STFP. Our most significant finding is that the basal forebrain ACh levels in the CBD-treated mice, but not their control counterparts, were positively correlated with mice\'s STFP memory performance.
CONCLUSIONS: This study indicates that CBD enhances STFP memory in mice. Specifically, those which respond to CBD by increasing the muscarinic-mediated ACh signaling perform better in their STFP memory.