抗生素耐药性是全球最重要的医疗保健挑战之一,每年导致全球数百万人死亡。这是由于滥用抗生素和缺乏新药开发造成的危机。放线菌构成一组革兰氏阳性细菌,以其基因组DNA中独特的高鸟嘌呤-胞嘧啶(GC)含量而闻名。这些微生物因其产生具有多种生物活性的多种次生代谢产物的能力而得到广泛认可。这些多才多艺的微生物在不同的生态系统中无处不在,包括土壤,淡水,海洋沉积物,在昆虫体内。最近的一项研究表明,社会性昆虫,比如蚂蚁,宿主这些细菌的不同阵列。在这项研究中,我们涉及从泰国各个地区收集的18种不同的蚂蚁物种中获得的总共72种放线菌菌株的分离和表征。利用16SrRNA基因分析,这些分离的放线菌被分为四个不同的属:霉菌属(2个分离株),小单孢菌(1个分离物),诺卡氏菌(8个分离株),和链霉菌(61个分离株)。在链霉菌菌株中,23个分离株对一组革兰氏阳性细菌表现出抗菌活性,包括枯草芽孢杆菌ATCC6633,表皮葡萄球菌ATCC12228,金黄色葡萄球菌ATCC25923,KocuriarhizophilaATCC9341和耐甲氧西林金黄色葡萄球菌(MRSA)DMST20646。此外,两个分离株对白色念珠菌TISTR5554显示抗真菌活性。基于16SrRNA基因序列相似性研究,这两个分离物,ODS25和ODS28被证明与lusitanus链霉菌NBRC13464T(98.07%)和卤链霉菌DSM41970T(97.28%)密切相关,分别。16SrRNA基因序列相似性低于98.65%的截止值表明其作为新型放线菌的潜力。这些发现强调了源自蚂蚁的放线菌作为新型抗菌剂的有价值的储库的潜力。
Antibiotic resistance is one of the most important global healthcare challenges and is responsible for the mortality of millions of people worldwide every year. It is a crisis attributed to misuse of antibiotics and a lack of new drug development. Actinomycetes constitute a group of Gram-positive bacteria known for their distinctive high guanine-cytosine (G+C) content in their genomic DNA. These microorganisms are widely recognized for their capability to generate a wide range of secondary metabolites with diverse biological activities. These versatile microorganisms are ubiquitous in diverse ecosystems, including soil, freshwater, marine sediments, and within the bodies of insects. A recent study has demonstrated that social insects, such as ants, host a diverse array of these bacteria. In this study, we involved the isolation and characterization of a total of 72 actinomycete strains obtained from 18 distinct ant species collected from various regions across Thailand. Utilizing 16S rRNA gene analysis, these isolated actinomycetes were classified into four distinct genera: Amycolatopsis (2 isolates), Micromonospora (1 isolate), Nocardia (8 isolates), and Streptomyces (61 isolates). Among the Streptomyces strains, 23 isolates exhibited antimicrobial activity against a panel of Gram-positive bacteria, including Bacillus subtilis ATCC 6633, Staphylococcus epidermidis ATCC 12228, Staphylococcus aureus ATCC 25923, Kocuria rhizophila ATCC 9341, and Methicillin-resistant Staphylococcus aureus (MRSA) DMST 20646. Additionally, two isolates displayed antifungal activity against Candida albicans TISTR 5554. Based on 16S rRNA gene sequence similarity studies, these two isolates, ODS25 and ODS28, were demonstrated to be closely related to Streptomyces lusitanus NBRC 13464T (98.07%) and Streptomyces haliclonae DSM 41970T (97.28%), respectively. The level of 16S rRNA gene sequence similarity below 98.65% cutoff indicates its potential as a novel actinomycete species. These findings underscore the potential of actinomycetes sourced from ants as a valuable reservoir of novel antimicrobials.