本研究旨在探讨紫菜种子(LSE)水提物改善大鼠模型创面愈合过程的能力。明胶,用柠檬酸从光滑猎犬鲨的皮肤中提取,用作软膏的支持材料。将动物分为四组,每组六只大鼠:未经处理的对照组,对照组用湿润烧伤膏(MEBO)治疗,用明胶凝胶治疗组,和用20mg/mLLSE强化的明胶凝胶处理组。酚类分析表明,LSE中的主要化合物是儿茶素(125μg/g)和奎尼酸(105μg/g)。体外抗氧化测试表明,LSE具有清除ABTS•自由基(IC50=0.22mg/mL)和抑制亚油酸氧化的活性。与用MEBO处理的大鼠相比,在未处理的大鼠的损伤组织中观察到抗氧化酶活性的显着下降以及硫代巴比妥酸反应性物质(TBARS)和炎症标记物的水平的增加。有趣的是,当用明胶凝胶处理受伤的组织时,这种趋势发生了明显的逆转。Further,通过用LSE富集明胶凝胶,CAT的水平,GPx和SOD活性显著增加了35、126和212%,分别,而TBARS水平降低了31%。这些结果与伤口收缩百分比和组织学分析一致,这表明富含LSE的明胶凝胶对再生受损组织的潜在作用。
This study aimed to investigate the ability of aqueous extract of Lepidium sativum seeds (LSE) to improve the wound healing process in rat models. The gelatin, extracted from the skin of smooth-hound shark using citric acid, was used as a support material for ointment. Animals were divided into four groups of six rats each: an untreated control group, a control group treated with Moist Exposed Burn Ointment (MEBO), a treated group with gelatin gel, and a treated group with gelatin gel fortified with 20 mg/mL LSE. Phenolics profile analysis showed that the major compounds in LSE were catechin (125 μg/g) and quinic acid (105 μg/g). In vitro antioxidant tests showed that LSE has interesting activities to scavenge ABTS•+ radicals (IC50 = 0.22 mg/mL) and inhibit the oxidation of linoleic acid. A significant decline in the antioxidant enzymes activities and an increase in the level of thiobarbituric acid reactive substances (TBARS) and inflammatory markers was observed within the injured tissues of the untreated rats compared to rats treated with MEBO. Interestingly, when the wounded tissue was treated with gelatin gel a remarkable reversal of this trend occurred. Further, by enrichment of gelatin gel with LSE, the levels of CAT, GPx and SOD activities significantly increased by 35, 126, and 212 %, respectively, whereas the TBARS level was reduced by 31 %. These results were consistent with the wound contraction percentage and histological analysis, which suggest the potential effect of LSE-enriched gelatin gels to regenerate damaged tissues.