■周围神经入侵(PNI)是指入侵,encasement,或肿瘤细胞在神经周围或通过神经渗透。各种恶性肿瘤,包括胰腺癌,头颈部肿瘤,和胆管癌,展示了PNI的特点。特别是,在头颈颅底肿瘤,如腺样囊性癌(ACC),PNI是导致手术切除不全和术后复发的重要因素。
■在具有PNI的ACC组织的情况下进行空间转录组和单细胞转录组测序,以鉴定靶向PNI的潜在探针。通过体内和体外实验验证了探针的功效。
■空间转录组和单细胞RNA测序揭示了ACCPNI区域内施万细胞的表型变化。肽探针是根据施万细胞在PNI区的抗原呈递特性设计的,其依赖于主要组织相容性复合物II(MHC-II)分子。体外和体内实验的成功验证证实,这些探针可以在PNI区域标记活的雪旺细胞,作为动态体内标记肿瘤侵入神经的工具。
■靶向施旺细胞\'MHC-II分子的肽探针有可能证明ACC患者中PNI的发生。
UNASSIGNED: Perineural invasion (PNI) refers to the invasion, encasement, or penetration of tumor cells around or through nerves. Various malignant tumors, including pancreatic cancer, head and neck tumors, and bile duct cancer, exhibit the characteristic of PNI. Particularly, in head and neck-skull base tumors such as adenoid cystic carcinoma (ACC), PNI is a significant factor leading to incomplete surgical resection and postoperative recurrence.
UNASSIGNED: Spatial transcriptomic and single-cell transcriptomic sequencing were conducted on a case of ACC tissue with PNI to identify potential probes targeting PNI. The efficacy of the probes was validated through in vivo and in vitro experiments.
UNASSIGNED: Spatial transcriptomic and single-cell RNA sequencing revealed phenotypic changes in Schwann cells within the PNI region of ACC. Peptide probes were designed based on the antigen-presenting characteristics of Schwann cells in the PNI region, which are dependent on Major Histocompatibility Complex II (MHC-II) molecules. Successful validation in vitro and in vivo experiments confirmed that these probes can label viable Schwann cells in the PNI region, serving as a tool for dynamic in vivo marking of tumor invasion into nerves.
UNASSIGNED: Peptide probes targeting Schwann cells\' MHC-II molecules have the potential to demonstrate the occurrence of PNI in patients with ACC.