在这项研究中,它的目的是确定芥子酸(SNP)的作用,一种含有抗氧化剂的多酚,抗炎和抗菌性能,关于万古霉素(VCM)引起的睾丸损伤,一种广泛使用的抗革兰氏阳性菌的抗生素。研究中总共使用了35只雄性SpragueDawley大鼠,分为五组:对照组,VCM,SNP,VCM+SNP10和VCM+SNP20。口服一周后,大鼠在七氟烷麻醉下安乐死。虽然VCM组的MDA水平显着增加,SNP给药抑制了MDA水平的增加。VCM导致GSH水平显著下降,SOD,CAT,大鼠睾丸组织中的GPx活性,而SNP给药增加了这些抗氧化剂水平。SNP降低VCM诱导的Nrf-2,HO-1和NQO1在睾丸组织中的mRNA表达水平,同时增加MAPK14,MAPK15,JNK的水平,P53、Apaf-1、Caspase-3、Caspase-6、Caspase-9和Beclin-1mRNA转录物水平。VCM组睾丸组织中Bax和NF-κB水平显著升高,而Bcl-2水平下降。VCM显著降低了大鼠精子活力,增加了受损精子的百分比。组织病理学结果显示,VCM引起基底膜的破坏和生精小管的解体,但是SNP管理保留了睾丸组织学。因此,VCM增加氧化应激,凋亡,和大鼠睾丸组织中的自噬,睾丸组织病理学改变,精子质量下降,而SNP降低了这些影响。
In this study, it was aimed to determine the effect of sinapic acid (SNP), a polyphenol with antioxidant, anti-inflammatory and antibacterial properties, on testicular damage caused by vancomycin (VCM), a widely used antibiotic against gram positive bacteria. A total of 35 male Sprague Dawley rats were used in the study, divided into five groups: control, VCM, SNP, VCM + SNP 10, and VCM + SNP 20. Following a week of oral administration, the rats were euthanized under sevoflurane anesthesia. While the VCM group had a significant increase in MDA levels, the SNP administration inhibited the increase in MDA levels. VCM led to a significant decrease in GSH levels, SOD, CAT, and GPx activity in the testicular tissue of rats, while SNP administration increased these antioxidant levels. SNP administration decreased the mRNA expression levels of VCM induced Nrf-2, HO-1, and NQO1 in testicular tissue while increasing the levels of MAPK14, MAPK15, JNK, P53, Apaf-1, Caspase-3, Caspase-6, Caspase-9, and Beclin-1 mRNA transcript levels. The VCM group showed a significant increase in Bax and NF-κB levels in testicular tissue, while Bcl-2 levels decreased. VCM significantly decreased sperm motility and increased the percentage of damaged sperm in rats. Histopathological results revealed that VCM caused disruption of basement membranes and disorganization of seminiferous tubules, but SNP administration preserved testicular histology. As a result, VCM increased oxidative stress, apoptosis, and autophagy in the testicular tissue of rats, altered testicular histopathology, and decreased sperm quality, while SNP decreased these effects.