背景与目的:粘蛋白通过多种机制参与肿瘤细胞的发育和生长。然而,唾液腺肿瘤中粘蛋白表达的研究是有限的,尤其是小唾液腺的样本。这项研究旨在调查和比较粘蛋白在次要和主要唾液腺起源的良性和恶性唾液腺肿瘤中的表达。材料和方法:使用特殊染色剂对中性粘蛋白(高碘酸希夫)进行染色,来自六个正常唾液腺和73个唾液腺肿瘤的组织内的唾液酸粘蛋白(AlcianBlue)和磺粘蛋白(AldehideFuschin),包括31个多形性腺瘤,27例粘液表皮样癌,和15例腺样囊性癌.半定量方法用于评估导管腔内的粘蛋白表达。唾液酸粘蛋白是所有唾液腺肿瘤中表达最多的粘蛋白,不管起源。结果:良性和恶性唾液腺肿瘤之间的粘蛋白表达存在显着差异,与粘液表皮样癌和腺样囊性癌相比,多形性腺瘤显示唾液粘蛋白的表达明显高出三倍(p=0.028)。与次要腺体相比,主要腺体的多形性腺瘤显示出42倍的唾液酸粘表达(p=0.000)。结论:与小腺体相比,大腺体多形性腺瘤中的唾液酸蛋白含量大大增加。唾液粘蛋白在良性和恶性唾液腺肿瘤中的差异表达表明在诊断交界性唾液腺肿瘤中的作用。
Background and Objectives: Mucin has been implicated via various mechanisms in the development and growth of tumour cells. However, mucin expression studies in salivary gland tumours are limited, especially with samples from minor salivary glands. This study aims to investigate and compare mucin expression in benign and malignant salivary gland tumours of minor and major salivary gland origins. Materials and Methods: Special stains were used to stain neutral mucin (Periodic acid Schiff),
sialomucin (Alcian Blue) and sulfomucin (Aldehyde Fuschin) within tissues from six normal salivary glands and 73 salivary gland tumours including 31 pleomorphic adenomas, 27 mucoepidermoid carcinomas, and 15 adenoid cystic carcinomas. A semi-quantitative approach was used to evaluate mucin expression within ductal lumens.
Sialomucin was the most expressed mucin in all salivary gland tumours, regardless of origin. Results: A significant difference was observed in the mucin expression between benign and malignant salivary gland tumours, as pleomorphic adenoma showed three times significantly higher expression of sialomucin compared to mucoepidermoid carcinoma and adenoid cystic carcinoma (p = 0.028). Pleomorphic adenomas of major glands showed 42 times significantly higher expression of
sialomucin compared to those of minor glands (p = 0.000). Conclusions:
Sialomucin content in pleomorphic adenomas of major glands was vastly increased compared to that in minor glands. Differential
sialomucin expression in benign and malignant salivary gland tumours suggests a role in diagnosing of borderline salivary gland tumours.