■千花羊肉美利奴羊是我国自主培育的肉羊新品种。2018年获国家批准,纳入国家畜禽遗传资源名录。千花肉羊具有典型肉畜品种的共同特点,早期生长发育快,产肉性能高。本研究的目的是探讨千花肉羊繁殖性状的全基因组关联。
■选择选育核心群体的千花肉羊为研究对象,GWAS对与生殖性状相关的基因进行了研究(单胎或双胞胎,出生体重,性成熟的年龄[以天为单位],断奶体重,以及从出生到断奶的每日增重)千花羊肉。
■我们的研究结果表明,检测到151个SNP位点,其中与出生体重和断奶体重相关的3个SNP占据了广泛基因组的很大一部分。初步获得的候选基因为SYNE1、SLC12A4、BMP2K、CAMK2D,IMMP2L,DMD,和BCL2。
■对与繁殖相关的五个性状(包括单胎或双胞胎,出生体重,性成熟时的年龄[以天为单位],断奶体重,从出生到断奶的每日体重增加)。这些候选基因的功能主要富集在核苷酸代谢,金属离子结合,催产素信号通路,和神经营养蛋白信号通路。
OBJECTIVE: Qianhua Mutton Merino sheep is a new breed of meat wool sheep cultivated independently in China. In 2018, it was approved by the state and brought into the national list of livestock and poultry genetic resources. Qianhua Mutton Merino sheep have the common characteristics of typical meat livestock varieties with rapid growth and development in the early stage and high meat production performance. The objective of this research is to investigate the Genome-wide association of the reproductive traits of Qianhua Mutton Merino sheep.
METHODS: Qianhua Mutton Merino sheep from the breeding core group were selected as the research object, genome-wide association analysis was conducted on genes associated with the reproductive traits (singleton or twins, birth weight, age [in days] for sexual maturity, weaning weight, and daily gain from birth to weaning) of Qianhua mutton merino.
RESULTS: Our study findings showed that 151 loci of single-nucleotide polymorphisms (SNPs) were detected, among which 3 SNPs related to birth weight and weaning weight occupied a significant portion of the wide genome. The candidate genes preliminarily obtained were SYNE1, SLC12A4, BMP2K, CAMK2D, IMMP2L, DMD, and BCL2.
CONCLUSIONS: We found 151 SNP loci for five traits related to reproduction (including singleton or twins, birth weight, age [in days] at sexual maturity, weaning weight, and daily weight gain from birth to weaning). The functions of these candidate genes were mainly enriched in nucleotide metabolism, metal ion binding, oxytocin signaling pathway, and neurotrophin signaling pathway.