本研究旨在探讨膳食中添加三种天然抗氧化剂对性激素水平的影响,酶和非酶抗氧化系统,和雄性尼罗罗非鱼睾丸的组织学变化,尼罗罗非鱼。将总共210只雄性尼罗罗非鱼分配到七个处理中(每个处理三个重复),初始重量为3.67g鱼-1。给鱼喂食实验饮食(32%粗蛋白),不补充作为对照或补充人参提取物(GE;0.2和0.4gGEkg-1饮食),藜麦提取物(TT;0.6和1.2gTTkg-1饮食),和椰枣花粉粒(DPPG;3和6gDPPGkg-1日粮)84天。结果显示,随着TT,黄体生成素水平显着增加,DPPG,补充GE使水平提高了22.9%,18.5%,和17.6%,分别。睾酮水平也随着TT1.2、GE0.4、TT0.6和DPPG6显著增加86.23%,64.49%,57.40%,和24.62%,分别。使用不同的膳食物质时,睾丸匀浆中的抗氧化剂状态显示硫代巴比妥酸反应性物质的水平显着降低。此外,谷胱甘肽含量降低,谷胱甘肽S-转移酶,谷胱甘肽过氧化物酶,过氧化氢酶,和超氧化物歧化酶活性在不同的膳食补充剂中以剂量依赖的方式显着增加。组织学评估显示,在所有治疗中,睾丸的组织学特征正常,GE中活跃的生精小管(%)增加,TT,和DPPG补充组,尤其是最高级别的。总之,GE的膳食补充剂,TT,DPPG提高了性激素水平,氧化还原状态,能提高尼罗罗非鱼雄性繁殖性能。
This study aimed to investigate the effect of dietary supplementation of three natural antioxidants on sex hormone levels, enzymatic and non-enzymatic antioxidant systems, and histological changes in the testes of male Nile tilapia, Oreochromis niloticus. A total of 210 male Nile tilapia were distributed into seven treatments (three replicates for each) with an initial weight of 3.67 g fish-1. The fish were fed experimental diets (32% crude protein) without supplementation as control or supplemented with ginseng extract (GE; 0.2 and 0.4 g GE kg-1 diet), Tribulus terrestris extract (TT; 0.6 and 1.2 g TT kg-1 diet), and date palm pollen grains (DPPG; 3 and 6 g DPPG kg-1 diet) for 84 days. The results revealed a significant increase in the luteinizing hormone level with TT, DPPG, and GE supplementation increased the levels by 22.9%, 18.5%, and 17.6%, respectively. The testosterone level also increased significantly with TT1.2, GE0.4, TT0.6, and DPPG6 by 86.23%, 64.49%, 57.40%, and 24.62%, respectively. The antioxidant status in the testis homogenate showed a significant decrease in the level of thiobarbituric acid-reactive substances when using different dietary substances. In addition, glutathione reduced contents, glutathione S-transferases, glutathione peroxidase, catalase, and superoxide dismutase activities significantly increased with different dietary supplementation in a dose-dependent manner. The histological evaluation revealed normal histological features of the testes in all treatments with increasing active seminiferous tubules (%) in GE, TT, and DPPG supplemented groups, especially with the highest levels. In conclusion, the dietary supplementation of GE, TT, and DPPG enhanced sex hormones level, redox status, and testis structure and could improve the male reproductive performance of Nile tilapia.