性别决定非常多样化,性染色体之间频繁的转换,在两栖动物中。在这些转变下,一些染色体更有可能被反复增选为性染色体,因为它们经常在分歧很大的类群中被观察到。然而,对密切相关群体的性染色体进化模式知之甚少。这里,我们检查了两只多刺青蛙的性染色体和性别决定,Nanoranaquadranus和Quasipaayei。我们对总共34名个体的基因分型测序(GBS)数据进行了分析,以确定特定性别的制造者。结果通过PCR验证。结果表明,1号染色体是两个物种中具有XY模式的同源性染色体。在一段时间内,这些密切相关的群体中的染色体在进化上是保守的。DMRT1基因被认为与两个远缘相关的多刺青蛙物种的同源性有关,作为推定的候选性别决定基因。携带DMRT1基因,1号染色体将在差异很大的无性系中独立选择用于性别确定。
Sex determination is remarkably diverse, with frequent transitions between sex chromosomes, in amphibians. Under these transitions, some chromosomes are more likely to be recurrently co-opted as sex chromosomes, as they are often observed across deeply divergent taxa. However, little is known about the pattern of sex chromosome evolution among closely related groups. Here, we examined sex chromosome and sex determination in two spiny frogs, Nanorana quadranus and Quasipaa yei. We conducted an analysis of genotyping-by-sequencing (GBS) data from a total of 34 individuals to identify sex-specific makers, with the results verified by PCR. The results suggest that chromosome 1 is a homologous sex chromosome with an XY pattern in both species. This chromosome has been evolutionarily conserved across these closely related groups within a period of time. The DMRT1 gene is proposed to be implicated in homology across two distantly related spiny frog species as a putative candidate sex-determining gene. Harboring the DMRT1 gene, chromosome 1 would have been independently co-opted for sex determination in deeply divergent groups of anurans.