间充质干细胞在组织修复过程中可以分化为特定的细胞系。使用激光和LED进行光生物调节用于治疗多种合并症,会干扰细胞增殖和活力,除了促进与所采用的物理参数相关的响应。评估和比较激光和LED对间充质细胞的影响,不同的能量剂量和不同的波长,除了生存能力和伤口闭合。来自人脂肪细胞的间充质干细胞用激光照射(能量为0.5J,2J和4J,波长为660nm和830nm),和LED(能量为0.5J,2J和4J,其中长度为630nm和850nm)。通过体外监测病变面积的减少来评估伤口闭合过程。通过用Hoechst和碘化丙啶标记物分析确定活力,活细胞和非活细胞的定量分别使用Shapiro-Wilk检验分析数据分布。使用Levene检验分析均匀性。使用双向ANOVA检验分析所使用的参数之间的比较。将T检验应用于与活力和病变面积相关的数据。对于LED光生物调节,只有630nm波长在24、48和72h中获得了显着结果(p=0,027;p=0,024;p=0,009)。与体外伤口闭合测试相关的结果表明,考虑到接近边缘所花费的时间,使用激光和LED的光生物调节均表现出显著的结果(p<0.05)。考虑到本研究的体外实验条件,可以得出结论,光生物调节的物理参数,比如能量和波长,在间充质干细胞中使用激光或LED,可以在细胞活力和伤口闭合中起潜在的作用。
Mesenchymal stem cells can differentiate into specific cell lineages in the tissue repair process. Photobiomodulation with laser and LED is used to treat several comorbidities, can interfere in cell proliferation and viability, in addition to promoting responses related to the physical parameters adopted. Evaluate and compare the effects of laser and LED on mesenchymal cells, with different energy doses and different wavelengths, in addition to viability and wound closure. Mesenchymal stem cells derived from human adipocytes were irradiated with laser (energy of 0.5 J, 2 J and 4 J, wavelength of 660 nm and 830 nm), and LED (energy of 0.5 J, 2 J and 4 J, where lengths are 630 nm and 850 nm). The wound closure process was evaluated through monitoring the reduction of the lesion area in vitro. Viability was determined by analysis with Hoechst and Propidium Iodide markers, and quantification of viable and non-viable cells respectively Data distributions were analyzed using the Shapiro-Wilk test. Homogeneity was analyzed using Levene\'s test. The comparison between the parameters used was analyzed using the Two-way ANOVA test. The T test was applied to data relating to viability and lesion area. For LED photobiomodulation, only the 630 nm wavelength obtained a significant result in 24, 48 and 72 h (p = 0,027; p = 0,024; p = 0,009). The results related to the in vitro wound closure test indicate that both photobiomodulation with laser and LED demonstrated significant results considering the time it takes to approach the edges (p < 0.05). Considering the in vitro experimental conditions of the study, it is possible to conclude that the physical parameters of photobiomodulation, such as energy and wavelength, with laser or LED in mesenchymal stem cells, can play a potential role in cell viability and wound closure.