硒酮,2-硒基-Nα,Nα,Nα-三甲基-L-组氨酸,是海洋鱼类中主要的有机硒化合物。为了表征硒酮在鱼类中的生物抗氧化功能,硒酮和其他硒化合物的积累,i.e.,亚硒酸钠和硒代甲硫氨酸,在红色海豹的肌肉和其他组织中。我们通过饲喂含有亚硒酸钠的1%干颗粒来饲养红sea鱼,硒代蛋氨酸,或硒酮体重每天两次,持续4周。之后,我们从硒补充剂中每天两次更换1%的正常商业干颗粒,持续1周,测定了总硒的组织分布。硒补充剂硒酮,硒代蛋氨酸,亚硒酸钠增强了白肌肉中硒的积累,肾,和肝胰腺与对照组比较。通过硒酮的饮食摄入,白肌肉中总硒浓度增加,心,肾,脾,脾肝胰腺,大脑,和血细胞在2周后的试验期间呈剂量依赖性。饮食中摄入硒酮以及亚硒酸钠和硒代甲硫氨酸可降低氧化还原电位(ORP)。硒酮在白肌和血细胞中的浓度通过硒酮摄入积累4周,而硒代蛋氨酸和亚硒酸钠的摄入并没有提高硒代蛋氨酸的浓度,这表明组织硒酮水平可能仅来自含硒酮的饮食。经计算,含有硒酮的人工饲料中硒酮的摄取因子在白肌中为0.0062,在血液中为4.0。总硒在血细胞和白肌肉中的半衰期估计为60天,在血液中为30天。
Selenoneine, 2-selenyl-Nα, Nα, Nα-trimethyl-L-histidine, is the major organic selenium compound in marine fish. To characterize biological antioxidant function of
selenoneine in fish, the accumulation of
selenoneine and other selenium compounds, i. e., sodium selenite and selenomethionine, in the muscle and other tissues of red seabream. We reared red seabream by feeding of 1% dry pellet containing of sodium selenite, selenomethionine, or selenoneine of body weight twice a day for 4 weeks. After that, we replaced to 1% of normal commercial dry pellet of body weight twice a day for 1 week from the selenium supplementation, and tissue distribution of total selenium was determined. Selenium supplementation with selenoneine, selenomethionine, and sodium selenite enhanced selenium accumulation in the white muscle, kidney, and hepatopancreas in comparison with the control group. By the dietary intake of selenoneine, total selenium concentrations were increased in the white muscle, heart, kidney, spleen, hepatopancreas, brain, and blood cells in a dose-dependent manner during the trials after 2 weeks. Dietary intake of selenoneine as well as sodium selenite and selenomethionine reduced oxidation-reduction potential (ORP).
Selenoneine concentrations in the white muscle and blood cells were accumulated for 4 weeks by the
selenoneine intake, whereas selenoneine concentration was not elevated by the intake of selenomethionine and sodium selenite, suggesting that tissue
selenoneine levels might be derived from only
selenoneine-containing diet. The uptake factor of
selenoneine from the artificial feed containing
selenoneine was calculated to be 0.0062 in the white muscle and 4.0 in the blood. The half-life of total selenium in the blood cells and white muscle were estimated to be 60 days in the white muscle and 30 days in the blood.