聚(ADP-核糖)聚合酶(PARP),将来自烟酰胺腺嘌呤二核苷酸(NAD+)的ADP-核糖的单体或聚合物转移到靶蛋白上,在DNA损伤修复中需要多个过程,细胞周期,发展,动物和植物的非生物胁迫。这里,未表征的水稻(Oryzasativa)OsPARP1,已被预测具有两个替代的OsPARP1mRNA剪接变体,研究了OsPARP1.1和OsPARP1.2。然而,双分子荧光互补表明,只有OsPARP1.1与OsPARP3对应物相互作用,表明OsPARP1.1是大米中的功能性蛋白质。在花发育过程中,OsPARP1优先在雄蕊的原始和花粉粒中表达。osparp1突变体和CRISPR植物的萌发延迟,表明DNA修复机制缺陷会损害早期种子萌发。突变体在营养生长过程中表现出正常的表型,但在正常条件下的结实率低于野生型植物。在I期至II期初,在雄性上皮细胞中检测到染色体桥和DNA片段。减数分裂II后,形成畸形的四分体或具有微核的四分体。同时,在胚囊发育中也发现了异常。总的来说,这些结果表明,OsPARP1在介导DNA损伤反应和配子体发育中起重要作用,在自然环境中对水稻产量至关重要。
Poly(ADP-ribose) polymerases (PARPs), which transfer either monomer or polymer of ADP-ribose from nicotinamide adenine dinucleotide (NAD+ ) onto target proteins, are required for multiple processes in DNA damage repair, cell cycle, development, and abiotic stress in animals and plants. Here, the uncharacterized rice (Oryza sativa) OsPARP1, which has been predicted to have two alternative OsPARP1 mRNA splicing variants, OsPARP1.1 and OsPARP1.2, was investigated. However, bimolecular fluorescence complementation showed that only OsPARP1.1 interacted with OsPARP3 paralog, suggesting that OsPARP1.1 is a functional protein in rice. OsPARP1 was preferentially expressed in the stamen primordial and pollen grain of mature stamen during flower development. The osparp1 mutant and CRISPR plants were delayed in germination, indicating that defective DNA repair machinery impairs early seed germination. The mutant displayed a normal phenotype during vegetative growth but had a lower seed-setting rate than wild-type plants under normal conditions. Chromosome bridges and DNA fragmentations were detected in male meiocytes at anaphase I to prophase II. After meiosis II, malformed tetrads or tetrads with micronuclei were formed. Meanwhile, the abnormality was also found in embryo sac development. Collectively, these results suggest that OsPARP1 plays an important role in mediating response to DNA damage and gametophyte development, crucial for rice yield in the natural environment.