目的:五味子乙素(SchB)已被证明具有抗炎和抗氧化特性,然而,其抗类风湿关节炎特性和潜在机制仍未被探索。这项研究评估了SchB在佐剂诱导的关节炎(AIA)大鼠中的潜力。
方法:通过将0.1mlCFA注射到大鼠的爪中诱导AIA,并给动物施用SchB(50mg/kg)28天。使用关节炎严重程度评估SchB的效果,血清氧化炎症水平,和代谢指标参数。
结果:SchB通过显著减少爪子肿胀和关节炎评分以及增加体重来缓解关节炎症状。此外,SchB减轻了包括白细胞介素-1β在内的氧化炎症标志物的水平,白细胞介素-6,肿瘤坏死因子α,核因子κB,转化生长因子β1,诱导型一氧化氮合酶和丙二醛,以及增加超氧化物歧化酶的水平,谷胱甘肽,Nrf2SchB也显著恢复了甘油三酯水平的改变,天冬氨酸转氨酶,乳酸,丙酮酸,磷酸烯醇丙酮酸羧化酶,葡萄糖,缺氧诱导因子-1α,血管内皮生长因子.此外,SchB显著减轻治疗的AIA大鼠中的p65表达。
结论:这项研究表明,SchB通过减少氧化应激来缓解AIA,炎症,和血管生成。
OBJECTIVE: Schisandrin B (Sch B) has been shown to possess anti-inflammatory and antioxidant properties, however, its antirheumatoid arthritis properties and potential mechanism remain unexplored. This study evaluated the potential of Sch B in adjuvant-induced arthritic (AIA) rats.
METHODS: AIA was induced by injecting 0.1 ml of CFA into the paw of rats and the animals were administered with Sch B (50 mg/kg) for 28 days. The effects of Sch B were evaluated using arthritis severity, serum levels of oxido-inflammatory, and metabolic index parameters.
RESULTS: Sch B eased arthritic symptoms by significantly reducing paw swelling and arthritic score and increased body weight gain. Moreover, Sch B alleviated the levels of oxido-inflammatory markers including interleukin-1 beta, interleukin-6, tumor necrosis factor alpha, nuclear factor kappa B, transforming growth factor β1, inducible nitric oxide synthase and malonaldehyde, as well as increased the levels of superoxide dismutase, glutathione, and Nrf2. Sch B also remarkably restored the altered levels of triglyceride, aspartate aminotransferase, lactic acid, pyruvate, phosphoenolpyruvate carboxylase, glucose, hypoxia inducible factor-1 alpha, and vascular endothelial growth factor. In addition, Sch B markedly alleviated p65 expression in the treated AIA rats.
CONCLUSIONS: This study suggests that Sch B alleviated AIA by reducing oxidative stress, inflammation, and angiogenesis.