■FTO基因属于非血红素Fe(II)和2氧戊二酸依赖性双加氧酶超家族。FTO基因的第一个内含子内的多态性已在各种人群中进行了检查,产生不同的发现。本研究旨在确定两种内含子多态性FTO30685T/G(rs17817449)和-23525T/A(rs9939609)对旁遮普邦肥胖风险的影响,印度。
■对671名无关参与者(肥胖=333和非肥胖=338)(年龄≥18岁)进行了基因型和生化分析。通过PCR-RFLP方法进行多态性的基因分型。然而,通过Sanger测序对50%的样品进行测序。
■两种FTO变体30685(TTvsGG:赔率比(OR),2.30;95%置信区间(CI),1.39-3.79)和-23525(TTvsAA:赔率比(OR),2.78;95%置信区间(CI),1.37-5.64)通过授予2次和3次,显示出肥胖的实质性风险,分别。通过逻辑回归分析显示,在隐性遗传模型下,变体30685T/G(rs17817449)和-23525T/A(rs9939609)(OR=2.29;95CI:1.47-3.57)和(OR=5.25;95%CI:2.68-10.28)均存在显着关联,分别。单倍型组合TA(30685;-23525)发展肥胖的4倍风险(P=0.0001)。在肥胖者中,30685T/G的G等位基因和-23525T/A的A-等位基因显示体重指数(BMI)的差异,腰围(WC),腰围与身高比(WHtR),收缩压(SBP),舒张压(DBP)和甘油三酯(TG)。
■本研究表明,FTO30685T/G(rs17817449)和-23525T/A(rs9939609)多态性对该人群中个体的肥胖易感性具有关键影响。
UNASSIGNED: FTO gene belongs to the non-heme Fe (II) and 2 oxoglutarate-dependent dioxygenase superfamily. Polymorphisms within the first intron of the FTO gene have been examined across various populations, yielding disparate findings.The present study aimed to determine the impact of two intronic polymorphisms FTO 30685T/G (rs17817449) and -23525T/A (rs9939609) on the risk of obesity in Punjab, India.
UNASSIGNED: Genotypic and biochemical analysis were done for 671 unrelated participants (obese=333 and non-obese=338) (age≥18 years). Genotyping of the polymorphisms was done by PCR-RFLP method. However, 50% of the samples were sequenced by Sanger sequencing.
UNASSIGNED: Both the FTO variants 30685 (TT vs GG: odds ratio (OR), 2.30; 95% confidence interval (CI), 1.39-3.79) and -23525 (TT vs AA: odds ratio (OR), 2.78; 95% confidence interval (CI), 1.37-5.64) showed substantial risk towards obesity by conferring it 2 times and 3 times, respectively. The analysis by logistic regression showed a significant association for both the variants 30685T/G (rs17817449) and -23525T/A (rs9939609) (OR=2.29; 95%CI: 1.47-3.57) and (OR=5.25; 95% CI: 2.68-10.28) under the recessive genetic model, respectively. The haplotype combination TA (30685; -23525) develops a 4 times risk for obesity (P=0.0001). Among obese, the G allele of 30685T/G and A- allele of -23525T/A showed variance in Body mass index (BMI), waist circumference (WC), waist-to-height ratio(WHtR), systolic blood pressure (SBP), diastolic blood pressure (DBP) and triglyceride(TG).
UNASSIGNED: The present investigation indicated that both the FTO 30685T/G (rs17817449) and -23525T/A (rs9939609) polymorphisms have a key impact on an individual\'s vulnerability to obesity in this population.