脊髓损伤(SCI)的初始机械损伤会引发进行性继发性损伤级联,这是一个复杂的过程,整合了多个系统和细胞。探索SCI后发生的分子和生物学过程改变对于治疗发展至关重要。SCI病变周围的头端和尾部区域之间的差异很少受到关注。这里,我们分析了损伤后头端和尾部位之间的差异表达基因,以确定SCI后这两个节段的生物学过程。我们确定了一组差异表达的基因,包括Col3a1,Col1a1,Dcn,Fn1,Kcnk3和Nrg1,在SCI后不同时间点的头端和尾区域之间。功能富集分析表明,这些基因参与了对机械刺激的反应,血管发育,和大脑发育。然后我们选择了Col3a1,Col1a1,Dcn,Fn1,Kcnk3和Nrg1用于定量实时PCR,Fn1用于免疫染色验证。我们的结果表明,不同的生物事件的变化富集在头端和尾部病变区域,为SCI的病理学提供新的见解。
The initial mechanical damage of a spinal cord injury (SCI) triggers a progressive secondary injury cascade, which is a complicated process integrating multiple systems and cells. It is crucial to explore the molecular and biological process alterations that occur after SCI for therapy development. The differences between the
rostral and caudal regions around an SCI lesion have received little attention. Here, we analyzed the differentially expressed genes between
rostral and caudal sites after injury to determine the biological processes in these two segments after SCI. We identified a set of differentially expressed genes, including Col3a1, Col1a1, Dcn, Fn1, Kcnk3, and Nrg1, between
rostral and caudal regions at different time points following SCI. Functional enrichment analysis indicated that these genes were involved in response to mechanical stimulus, blood vessel development, and brain development. We then chose Col3a1, Col1a1, Dcn, Fn1, Kcnk3, and Nrg1 for quantitative real-time PCR and Fn1 for immunostaining validation. Our results indicate alterations in different biological events enriched in the
rostral and caudal lesion areas, providing new insights into the pathology of SCI.