产生胞外多糖(EPS)的双歧杆菌,特别是动物双歧杆菌亚种。乳酸菌株,在功能食品工业中用作有希望的益生菌,具有据称的有益效果。我们使用了三个动物芽孢杆菌亚种的等基因菌株。乳酸,具有不同的EPS产生表型(黏液粘性和非粘性),为了确定它们在鼠胃肠道转运中存活的能力,以及评估它们在改善化学诱导结肠炎模型临床结局中的作用。在干预研究过程中,三株均能在C57BL/6J小鼠肠道中存活。此外,在治疗结束时,用各向异性应变治疗的动物组的疾病活动指数(DAI)显着低于安慰剂组的DAI。然而,三个菌株之间没有发现显着差异。几个免疫参数的分析,如在肠系膜淋巴结计数的血浆和淋巴细胞群体中定量的TNFα和IL-10,在四个实验动物组中显示出一些显着差异。值得注意的是,证明了在肠系膜淋巴结中增加调节性T细胞的更高能力,表明该菌株在粘膜水平调节炎症反应的能力更高。我们的数据表明,动物芽孢杆菌亚种的菌株。产生赋予粘液样表型的EPS的乳酸可能代表有希望的候选者,以针对肠道炎症过程进行进一步研究。
Exopolysaccharide (EPS)-producing bifidobacteria, particularly Bifidobacterium animalis subsp. lactis strains, are used in the functional food industry as promising probiotics with purported beneficial effects. We used three isogenic strains of B. animalis subsp. lactis, with different EPS producing phenotypes (mucoid-
ropy and non-
ropy), in order to determine their capability to survive the murine gastrointestinal tract transit, as well as to evaluate their role in improving clinical outcomes in a chemically-induced colitis model. The three strains were able to survive in the intestinal tract of C57BL/6J mice during the course of the intervention study. Furthermore, the disease activity index (DAI) of the animal group treated with the
ropy strain was significantly lower than of the DAI of the placebo group at the end of the treatment. However, no significant differences were found among the three strains. The analysis of several immune parameters, such as TNFα and IL-10 quantified in blood plasma and lymphocyte populations enumerated in mesenteric nodes, showed some significant variations among the four experimental animal groups. Remarkably, a higher capability of the
ropy strain to increase regulatory T-cells in mesenteric lymphoid nodes was demonstrated, suggesting a higher ability of this strain to regulate inflammatory responses at mucosal level. Our data indicate that strains of B. animalis subsp. lactis producing EPS that confer a mucoid-
ropy phenotype could represent promising candidates to perform further studies targeting intestinal inflammatory processes.