从在Hattar工业区受废水影响的土壤中生长的抗重金属大麻植物的根际中分离出一种产生色素的沙雷氏菌。哈里普尔,巴基斯坦。这里,我们报告了这种细菌的基因组序列,根据使用OrthoANI分类方案的全基因组比较,已将其鉴定为线虫沙雷氏菌。该细菌表现出不同的性状,包括促进植物生长,抗菌,生物修复,和污染物耐受能力,包括金属耐受性,偶氮染料降解,布洛芬降解,等。观察到促进植物生长的外切酶产生以及磷酸盐溶解特性。磷酸盐溶解的基因,铁载体生产,除其他工业上重要的酶如腈水解酶和脂肪酶外,还鉴定了几丁质的破坏。还分析了整个基因组中高价值化学物质的次级代谢物产生装置。然后将抗生素抗性基因的数量进行计算机分析,通过与抗生素抗性基因和CAR数据库的匹配。这是来自污染环境的线虫基因组的第一份报告。这可以大大有助于理解污染耐受性,抗生素耐药性,与线虫有关,生物农药的生产,以及它们在促进植物生长中的作用。
A pigment-producing species of Serratia was isolated from the rhizosphere of a heavy metal resistant Cannabis sativa plant growing in effluent-affected soil of Hattar Industrial Estate, Haripur, Pakistan. Here, we report the genome sequence of this bacterium, which has been identified as Serratia nematodiphila on the basis of whole genome comparison using the OrthoANI classification scheme. The bacterium exhibited diverse traits, including plant growth promotion, antimicrobial, bioremediation, and pollutant tolerance capabilities including metal tolerance, azo dye degradation, ibuprofen degradation, etc. Plant growth-promoting exoenzyme production as well as phosphate solubilisation properties were observed. Genes for phosphate solubilisation, siderophore production, and chitin destruction were identified in addition to other industrially important enzymes like nitrilase and lipase. Secondary metabolite producing apparatus for high value chemicals in the whole genome was also analysed. The number of antibiotic resistance genes was then profiled in silico, through a match with Antibiotic Resistant Gene and CAR database. This is the first report of a S. nematodiphila genome from a polluted environment. This could significantly contribute to the understanding of pollution tolerance, antibiotic resistance, association with nematodes, production of bio-pesticide, and their role in plant growth promotion.