背景:基于兔视网膜的组织学检查和玻璃体细胞因子的改变,探讨钕:钇铝石榴石(Nd:YAG)激光玻璃体溶解术的安全性。
方法:九只雄性新西兰兔在左眼中接受了10mJx500脉冲的Nd:YAG激光玻璃溶解,而右眼用作对照。眼内压,彩色眼底摄影,和之前测量的B超,以及1天,4周,Nd:YAG激光玻璃体溶解后12周。将三只兔子安乐死1天,4周,治疗后12周,分别。使用末端脱氧核苷酸转移酶dUTP缺口末端标记(TUNEL)染色和苏木精-伊红(H&E)染色来寻找视网膜中的病理变化。采用酶联免疫吸附试验(ELISA)检测血管内皮生长因子(VEGF)和一些炎性细胞因子的表达,包括干扰素诱导蛋白10(IP-10),玻璃体液中的单核细胞趋化蛋白1(MCP-1)和白细胞介素6(IL-6)。还测量了玻璃体液中的抗坏血酸(AsA)和总反应性抗氧化剂潜力(TRAP)。
结果:Nd:YAG激光玻璃溶解后,VEGF的水平,IP-10,MCP-1,IL6,AsA,玻璃体液中的TRAP无明显变化(P>0.05)。视网膜组织没有可检测到的病理变化,未发现凋亡信号。
结论:家兔耐受Nd:YAG激光玻璃体溶解,对视网膜组织或玻璃体微环境无明显影响。
BACKGROUND: To explore the safety of Neodymium:Yttrium-aluminum-garnet (Nd:YAG) laser vitreolysis based on the histological examination of the retina and the alteration of vitreous cytokines in the rabbits.
METHODS: Nine male New Zealand rabbits underwent Nd:YAG laser vitreolysis of 10 mJ x 500 pulses in the left eyes, while the right eyes were used as controls. Intraocular pressure, color fundus photography, and ultrasound B scan were measured before, as well as 1 day, 4 weeks, and 12 weeks after Nd:YAG laser vitreolysis. Three rabbits were euthanized 1 day, 4 weeks, and 12 weeks after treatment, respectively. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and hematoxylin-eosin (H&E) staining were used to look for pathological changes in the retina. An enzyme-linked immunosorbent assay (ELISA) was utilized to detect the expression of vascular endothelial growth factor (VEGF) and some inflammatory cytokines, including interferon inducible protein 10 (IP-10), monocyte chemoattractant protein 1 (MCP-1) and interlenkin 6 (IL-6) in the vitreous humor. The ascorbic acid (AsA) and total reactive antioxidant potential (TRAP) in the vitreous humor were also measured.
RESULTS: Following Nd:YAG laser vitreolysis, the levels of VEGF, IP-10, MCP-1, IL6, AsA, and TRAP in the vitreous humor did not change substantially (P > 0.05). There were no detectable pathological changes in the retinal tissues, and no apoptotic signal was found.
CONCLUSIONS: Rabbits tolerate Nd:YAG laser vitreolysis without observable impact on retinal tissue or the microenvironment of the vitreous.