■这项研究的目的是解开关岭牛的遗传性状,精确定位有利于肌肉生长的基因,为当地牛种遗传多样性的保持和重要经济性状调控机制的进一步分析奠定基础。
■在这项研究中,我们使用IlluminaHiSeqcBo测序平台对贵州3只关岭牛的全基因组进行了测序。And,采用高多重PCR技术检测其他55头关岭牛的高质量SNP位点。
■我们的研究确定了166,411个非同义SNP(nsSNP)和42,423个插入和缺失(indel)。通过SNP注释,基因功能富集分析,与西门塔尔相比,安格斯,和利木赞牛,我们确定了六个基因(LEPR,AKAP9,SIX4,SPIDR,PRG4,FASN)对肉质性状有潜在影响,在肌肉生长中起关键作用,脂肪代谢,和身体支持。我们还检查了关岭牛的七个SNP位点的多态性,发现所有七个位点均处于Hardy-Weinberg平衡。
■这些发现表明,这些基因位点在关岭牛种群中稳定且广泛存在。我们的研究为将来关岭牛的遗传增强和品种鉴定奠定了基础。
UNASSIGNED: The objective of this study was to unravel the genetic traits of Guanling cattle, pinpoint genes advantageous for muscle growth, and lay a foundation for the preservation of genetic diversity and further analysis of regulation mechanism of important economic traits in local cattle breed.
UNASSIGNED: In this study, we sequenced the whole genome of 3 Guanling cattle in Guizhou province using the Illumina HiSeq cBo sequencing platform. And, high- multiplex PCR technology was employed to detect high-quality SNP sites of other 55 Guanling cattle.
UNASSIGNED: Our study identified 166,411 non-synonymous SNPs (nsSNPs) and 42,423 insertions and deletions (indels). Through SNP annotation, gene function enrichment analysis, and comparing with Simmental, Angus, and Limousin cattle, we identified six genes (LEPR, AKAP9, SIX4, SPIDR, PRG4, FASN) which are potentially influential on meat quality traits, playing crucial roles in muscle growth, fat metabolism, and bodily support. We also examined polymorphisms at seven SNP sites in Guanling cattle and found that all seven were in Hardy-Weinberg equilibrium.
UNASSIGNED: These findings suggested that these gene sites are stable and widespread in the Guanling cattle population. Our research lays the groundwork for future genetic enhancement and variety identification of Guanling cattle.