BACKGROUND: This study evaluates the change in an MRI of the proximal metacarpal region in a group of sport horses that returned to work. This retrospective analysis evaluated 18 limbs represented by 17 horses.
RESULTS: The hyperintense signal within the dorsal collagenous part of the proximal suspensory ligament (PSL) on T1W/T2*W GRE sequences decreased or stayed the same in the majority of cases. The hyperintense STIR signal within the dorsal collagenous part of the PSL resolved in the majority of the patients, and the third metacarpal bone (McIII) hyperintense STIR signal resolved in all patients. The dorsal margin irregularity of the PSL stayed the same, and McIII sclerosis and resorption of the palmar margin of McIII stayed the same in the majority of cases. McIII hyperintense STIR signal resolution carries a broad time range, with a mean of 94 days and a range of 47-202 days.
CONCLUSIONS: Complete normalization of the dorsal collagenous part of the PSL does not appear necessary for a return to soundness, but a resolution of the McIII hyperintense STIR signal is expected for horses returning to soundness. A rescan period of 120 days for the proximal metacarpal region is suggested. In addition, there was no significant change in the size of the PSL between the initial and final MRI.

UNASSIGNED: To quantitatively analyze the dimensions of the gap between the intraocular lens (IOL) and the posterior capsule using microscope-integrated optical coherence tomography (MIOCT).
UNASSIGNED: Institutional.
UNASSIGNED: Prospective, interventional.
UNASSIGNED: A total of 105 eyes of 105 consecutive patients planned for phacoemulsification with IOL implantation were enrolled. At the end of surgery, the gap between the IOL and the posterior capsule (IOL-PC gap) was measured using MIOCT (RESCAN). The same gap was reassessed at 1 week follow-up visit using the Visante-OCT system. The cases were sub-grouped based on the grade of cataract, IOL material, size and design and were analyzed to look for any difference in the IOL-PC gap both intraoperatively and postoperatively.
UNASSIGNED: The IOL-PC gap could be successfully measured intra-operatively in all cases, using the RESCAN. The mean gap at the end of phacoemulsification was 385.08±264.58 µm, and at one week follow up this was 120.83± 95.13 µm.
UNASSIGNED: MIOCT may be successfully used to quantify the dimensions of the gap between the IOL and the posterior capsule. It can be used as a potential tool to further understand the dynamics of the IOL-PC space and to assess the impact of various IOL and non-IOL parameters implicated in the causation of posterior capsule opacification.

To measure the impact of different scanning patches on the accuracy (trueness and precision) of an intraoral scanner (IOS).
A typodont was digitized using an industrial optical scanner (GOM Atos Q 3D 12 M) to obtain a reference mesh. The typodont was scanned using an IOS (TRIOS 3). Three groups were generated based on the rescan areas created: no mesh holes (G0 group), 3 mesh holes distributed on the digital scan (G1 group), and 3 mesh holes located on the left quadrant of the digital scan (G2 group). In the G0 group, a digital scan was completed following the manufacturer\'s scanning protocol. In the G1 group, a digital scan was obtained following the same protocol as G0 group. Three 12-mm diameter holes were created in the occlusal surfaces of the left second first molar, incisal edges of the central incisors, and right first molar of the digital scan using the IOS software. In the G2 group, a digital scan was obtained following the same protocol as G0 group. Three 12-mm diameter holes in the digital scan were created in the occlusal surface of the left first molar and left second and first premolars using the IOS software program. The discrepancy between the control and the experimental digital scans was measured using the root mean square calculation. The Kolmogorov-Smirnov test demonstrated that data were normally distributed. One-way ANOVA followed by post hoc multiple comparison Bonferroni test were used to analyze the data (α = .05).
Trueness values ranged from 15 to 26 μm and the precision ranged from 21 to 150 μm. Significant differences in trueness mean values were found among the groups tested (F(2, 42) = 6.622, P = .003); the Bonferroni test indicated significant mean differences between the G0 and G2 groups (mean difference=0.11, SE=0.003, and P = .002). For precision evaluation, significant precision differences were found between the groups tested (F(2, 39)=9.479, P < .001); the Bonferroni test revealed significant precision differences between G0 and G2 groups (mean difference=-0.12, SE=0.030, and P = .001).
Rescanning mesh holes and stitching procedures decreased the trueness and precision of the IOS tested; furthermore, the number and dimensions of mesh holes rescanned represented an important factor that influenced the scanning accuracy of IOS tested.
It is a fundamental procedure obtaining intraoral digital scans without leaving mesh holes, so the rescanning techniques are minimized and, therefore, the scanning accuracy of the intraoral scanner tested is maximized.

A major goal for biologists is to understand the connection between genes and phenotypic traits, and genetic mapping in experimental populations remains a powerful approach for discovering the causal genes underlying phenotypes. For genetic mapping, the process of genotyping was previously a major rate-limiting step. Modern sequencing technology has greatly improved the resolution and speed of genetic mapping by reducing the time, labor, and cost per genotyping marker. In addition, the ability to perform genotyping-by-sequencing (GBS) has facilitated large-scale population genetic analyses by providing a simpler way to survey segregating genetic variation in natural populations. Here we present two protocols for GBS, using the Illumina platform, that can be applied to a wide range of genotyping projects in different species. The first protocol is for genotyping a subset of marker positions genome-wide using restriction digestion, and the second is for preparing inexpensive paired-end whole-genome libraries. We discuss the suitability of each approach for different genotyping applications and provide notes for adapting these protocols for use with a liquid-handling robot.

OBJECTIVE: To assess the added value of intraoperative optical coherence tomography (iOCT) in evaluating graft adhesion and graft interface in patients undergoing descemet\'s stripping automated endothelial keratoplasty (DSAEK).
METHODS: This is a prospective single-center case series comprising 8 eyes of 8 patients consecutively scheduled for DSAEK surgery. iOCT imaging was performed after insertion of the graft, after pressurizing the eye, and at the end of surgery (three images per surgery). At each stage of surgery, corneal thickness and the widest gap between the recipient and the graft (i.e., maximal interface width) were measured using an image processing tool. Follow-up measurements were taken at 1 day, 3 and 6 months, post-operatively.
RESULTS: Imaging was performed in 21 of 24 scheduled imaging intervals, and required little to no additional surgical time. At the end of surgery, iOCT showed persisting interfaces in six cases. One case showed a full graft detachment necessitating surgical intervention.
CONCLUSIONS: Real-time iOCT is a safe, efficient, and useful tool in assessing graft adherence in DSAEK surgery. With adequate analysis software, iOCT has the potential to be a paradigm-shifting development in posterior lamellar surgery and could aid the clinician in further lowering the rates of graft dislocation after DSAEK.

BACKGROUND: Susceptibility-weighted imaging (SWI) visualizes even small cerebral veins and might, therefore, be valuable in monitoring neurological diseases affecting cerebral veins. Since it is generally difficult to evaluate individual results of quantitative MRI measurements, an automatic approach would be highly appreciated to assist the diagnostic process. The aim of this study was to evaluate the rescan and reanalysis reliability using an automatic venous volumetric approach based on SWI in healthy controls.
METHODS: SWI was performed in ten healthy controls undergoing MRI examinations using a 32-channel head coil at 3 T five times on five different days. To test for rescan and reanalysis variability, the deep cerebral vein volume was quantified using ANTs and SPM8.
RESULTS: Total volumes of cerebral deep veins measured during five MRI scans in ten individuals (n = 50 scans) showed intra-individual volume changes ranging from 0.07 to 1.03 ml (mean variability = 10.2 %). Automatic reanalyses revealed exactly the same results in all scans.
CONCLUSIONS: Automatic SWI-based cerebral vein volumetry shows acceptable rescan-and excellent reanalyses-reliability in healthy volunteers. Therefore, this approach might be beneficial in intra-individual follow-up studies of neurological diseases affecting the cerebral venous system.

BACKGROUND: \"Watch, wait, and rescan\" (WWR) has an established place as a successful management option for a significant proportion of vestibular schwannomas (VS) as an alternative to microsurgical removal or stereotactic radiotherapy. VS may grow slowly and continuously, followed by stagnation or even shrinkage. We present two case reports of spontaneous shrinkage of VS along with a review of the literature.
METHODS: A 29-year-old female presented with a progressive history of visual blurring and intermittent diplopia over 2 months. A 29 mm of maximum intracranial diameter (ICD) VS with secondary obstructive hydrocephalus was diagnosed. The patient underwent a ventriculo-peritoneal shunt with resolution of her symptoms and opted for initial WWR management. Interval scanning between 2007 and 2014 showed progressive reduction in the maximum ICD together with reduction in the degree of central tumor enhancement. Maximum ICD at most recent follow up was 22 mm. A 28-year-old female was referred with right sensorineural deafness. A right VS of maximum ICD of 27 mm was diagnosed. Initial WWR management was planned after discussion. Serial imaging showed an initial increase in the size of the tumor followed by progressive reduction in size. The most recent follow up showed a maximum ICD of 20 mm.
CONCLUSIONS: Early WWR management can be associated with spontaneous shrinkage of VS over time. Prospective clinical study of larger numbers of such cases using the UK VS database may help to identify predictive factors for the spontaneous regression of VS.