我们的环境越来越受到各种分子的污染,其中一些被认为是内分泌干扰物。金属和邻苯二甲酸盐,源自工业活动,农业实践,或消费品,是此类污染物的突出例子。我们通过实验研究了重金属镉和邻苯二甲酸盐DEHP对蛾夜蛾的影响。更具体地说,幼虫在实验室条件下饲养,他们暴露于被两种剂量的镉污染的饮食中,浓度为62.5µg/g或125µg/g,两剂DEHP,分别为100ng/g和10µg/g,或者两种化合物的低剂量和高剂量的组合,与对照组进行比较。我们的发现表明,镉延迟了从幼虫到成虫的发育过渡。值得注意的是,镉和DEHP的结合加剧了这种延迟,突出协同效应。相比之下,单独DEHP不影响幼虫发育。此外,我们观察到镉暴露,无论是单独还是与DEHP结合,导致所有幼虫阶段的质量较低。然而,成年后暴露于镉的个体最终达到了与其他群体相似的质量。有趣的是,虽然我们的结果没有显示出任何的孵化成功的治疗效果,镉处理组的成人死亡率较高.这表明,虽然蛾可能优先考虑繁殖成功,他们在成年阶段的生存受到镉暴露的影响。总之,我们的研究证明了镉对发育的影响,质量,和成虫的生存,与DEHP结合使用时显示出协同作用。这些结果证实镉是一种内分泌干扰物,即使是低剂量.这些见解强调了了解低剂量污染物如镉和DEHP的毒理学影响的重要性,无论是单独还是组合。
Our environment is increasingly polluted with various molecules, some of which are considered endocrine disruptors. Metals and phthalates, originating from industrial activities, agricultural practices, or consumer products, are prominent examples of such pollutants. We experimentally investigated the impacts of the heavy metal cadmium and the phthalate DEHP on the moth Spodoptera littoralis. More specifically, larvae were reared in laboratory conditions, where they were exposed to diets contaminated with either two doses of cadmium at concentrations of 62.5 µg/g or 125 µg/g, two doses of DEHP at 100 ng/g and 10 µg/g, or a combination of both low and high doses of the two compounds, with a control group for comparison. Our findings indicate that cadmium delays the developmental transition from larva to adult. Notably, the combination of cadmium and DEHP exacerbated this delay, highlighting a synergistic effect. In contrast, DEHP alone did not affect larval development. Additionally, we observed that cadmium exposure, both alone and in combination with DEHP, led to a lower mass at all larval stages. However, cadmium-exposed individuals that reached adulthood eventually reached a similar mass to those in other groups. Interestingly, while our results did not show any effect of the treatments on hatching success, there was a higher adult mortality rate in the cadmium-treated groups. This suggests that while moths may prioritize reproductive success, their survival at the adult stage is compromised by cadmium exposure. In conclusion, our study demonstrates the impact of cadmium on the development, mass, and adult survival of moths, and reveals synergistic effects when combined with DEHP. These results confirm cadmium as an endocrine disruptor, even at low doses. These insights underscore the importance of understanding the toxicological effects of low doses of pollutants like cadmium and DEHP, both individually and in combination.