背景:再生牙科旨在增强口腔组织和器官的结构和功能。现代组织工程利用基于细胞和基因的疗法来推进传统治疗方法。研究已经证明了间充质干细胞(MSCs)在再生牙科中的潜力,随着一些进入临床试验。这篇综述全面审查了利用MSC进行各种治疗应用的动物研究。此外,它旨在弥合相关发现与MSC疗法的实际实施之间的差距,提供从临床前研究过渡到临床应用所涉及的挑战和转化方面的见解。
结论:为了实现这一目标,我们专注于与牙髓牙本质有关的协议和成就,牙槽骨,在动物和临床研究中使用牙科来源的MSCs进行牙周再生。各种类型的MSC,包括牙齿来源的细胞,骨髓干细胞,和脐带干细胞,被用于根管,牙周缺损,插座保存,和鼻窦提升手术。这些结果包括显著的硬组织重建,功能性纸浆再生,根伸长,牙周膜形成,和牙骨质沉积。然而,基于细胞的牙齿和牙周组织再生治疗仍处于早期阶段。个性化医学对干细胞疗法的需求不断增加,这凸显了科学家和负责任的组织需要制定符合良好生产规范的标准化治疗方案。确保高重现性,安全,和成本效益。
结论:再生牙科中的细胞疗法代表了一个不断发展的行业,具有巨大的好处和独特的挑战,因为它致力于建立可持续的,长期的,和有效的口腔组织再生解决方案。
BACKGROUND: Regenerative dentistry aims to enhance the structure and function of oral tissues and organs. Modern tissue engineering harnesses cell and gene-based therapies to advance traditional treatment approaches. Studies have demonstrated the potential of mesenchymal stem cells (MSCs) in regenerative dentistry, with some progressing to clinical trials. This review comprehensively examines animal studies that have utilized MSCs for various therapeutic applications. Additionally, it seeks to bridge the gap between related findings and the practical implementation of MSC therapies, offering insights into the challenges and translational aspects involved in transitioning from preclinical research to clinical applications.
CONCLUSIONS: To achieve this objective, we have focused on the protocols and achievements related to pulp-dentin, alveolar bone, and periodontal regeneration using dental-derived MSCs in both animal and clinical studies. Various types of MSCs, including dental-derived cells, bone-marrow stem cells, and umbilical cord stem cells, have been employed in root canals, periodontal defects, socket preservation, and sinus lift procedures. Results of such include significant hard tissue reconstruction, functional pulp regeneration, root elongation, periodontal ligament formation, and cementum deposition. However, cell-based treatments for tooth and periodontium regeneration are still in early stages. The increasing demand for stem cell therapies in personalized medicine underscores the need for scientists and responsible organizations to develop standardized treatment protocols that adhere to good manufacturing practices, ensuring high reproducibility, safety, and cost-efficiency.
CONCLUSIONS: Cell therapy in regenerative dentistry represents a growing industry with substantial benefits and unique challenges as it strives to establish sustainable, long-term, and effective oral tissue regeneration solutions.