背景:据报道,槲皮素对广泛的活动至关重要,包括减轻炎症,血小板聚集,毛细管通透性,和脂质过氧化。然而,槲皮素在妊娠期高血压疾病中的作用,没有完全理解。
方法:通过降低子宫灌注压(RUPP)建立大鼠妊娠期高血压模型。槲皮素通过管饲法给药。使用CODA6BP系统测量收缩压(SBP)和舒张压(DBP)。内皮素-1(ET-1)的血浆浓度,可溶性fms样酪氨酸激酶-1(sFlt-1),使用酶联免疫吸附测定试剂盒检测血管内皮生长因子(VEGF)。通过RT-PCR和Western印迹测定ET-1和内皮素-1A型受体(ETAR)的mRNA和蛋白水平。ETAR拮抗剂BQ-123通过渗透微型泵进行。
结果:在RUPP诱导的大鼠中,槲皮素治疗降低SBP和DBP,胎儿再吸收百分比,血浆ET-1和sFlt-1浓度,ET-1和ETAR水平,但增加胎儿体重和VEGF表达。BQ-123给药减弱SBP和DBP,抑制了致命的吸收百分比,并增加了RUPP大鼠的胎儿体重。
结论:槲皮素通过调节ET-1和ETAR减轻RUPP诱导的孕鼠高血压。
BACKGROUND: Quercetin was reported to be crucial for a broad range of activities, including attenuating inflammation, platelet aggregation, capillary permeability, and lipid peroxidation. However, the effect of quercetin in hypertension during pregnancy, was not fully understood.
METHODS: The model of hypertension in pregnancy was established in rats by reduced uterine perfusion pressure (RUPP). Quercetin was administrated by gavage. Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured using the CODA 6 BP system. Plasma concentrations of Endothelin-1 (ET-1), soluble fms-like tyrosine kinase-1 (sFlt-1), and vascular endothelial growth factor (VEGF) were detected using enzyme-linked immunosorbent assay kits. The mRNA and protein levels of ET-1 and endothelin-1 type A receptor (ETAR) were determined by RT-PCR and Western blotting. The ETAR antagonist BQ-123 was performed by osmotic minipumps.
RESULTS: In RUPP induced rats, quercetin treatment decreased SBP and DBP, fetal resorptions percentage, plasma ET-1 and sFlt-1 concentrations, ET-1 and ETAR levels, but increased fetal body weight and VEGF expression. BQ-123 administration attenuated SBP and DBP, suppressed fatal resorptions percentage, and increased fetal body weight of RUPP rats.
CONCLUSIONS: Quercetin attenuates RUPP induced hypertension in pregnant rats through the regulation of ET-1 and ETAR.