本研究代表了成功使用金属氧化物纳米颗粒修复重金属的新证据。对氧化锌纳米颗粒(ZnONP)和氧化铜纳米颗粒(CuONP)进行了评估,以减轻菜豆幼苗和植物中的镉(Cd)毒性。监测生理和代谢参数可以阐明Cd对豆类产生植物毒性作用的机制和过程。P.vulgaris幼苗的反应是NP剂量依赖性的(10mg/L,50mg/L,100mg/L,和200mg/L)。同样,施加的浓度触发了生长中的植物在长度和生物量方面的差异响应。我们的生理数据允许选择100毫克/升作为最合适的浓度,以避免任何植物毒性的风险。首次在Cd胁迫下豆幼苗的胚轴中比较了ZnONP和CuONP的作用机制。两种NP都能够减少过氧化氢(H2O2)的过度生成。它们还通过增强ROS清除酶能力起作用,和抗氧化酶CAT的活性,APX,GPOX,GPX,和GR,并抑制产生ROS的酶如GOX和NOX的活性。NP的另一个机制作用包括氧化还原酶Trx的调节,NTR,Fd,和FNR在细胞内稳态中进化并在细胞中维持降低的状态。一起来看,ZnONP触发了更重要的代谢调节,从而减轻了Cd引起的氧化损伤。
The present study represents new evidence of the successful use of metal oxide nanoparticles in the remediation of heavy metals. Zinc oxide nanoparticles (ZnO NP) and copper oxide nanoparticles (CuO NP) were assessed to alleviate cadmium (Cd) toxicity in Phaseolus vulgaris L. seedlings and plants. Monitoring physiological and metabolic parameters allowed to elucidate Cd mechanism and process whereby it exerts phytotoxic effects on bean. The response of P. vulgaris seedlings is NP dose-dependent (10 mg/L, 50 mg/L, 100 mg/L, and 200 mg/L). Similarly, applied concentrations triggered a differential response of growing plants in terms of length and biomass. Our physiological data allowed to select 100 mg/L as the most appropriate concentration to apply, in order to avoid any risk of phytotoxicity. The regulatory mechanisms by which ZnO NP and CuO NP act are for the first time compared in the embryonic axes of bean seedlings under Cd stress. Both NP were able to reduce the hypergeneration of hydrogen peroxide (H2O2). They also acted via enhancing ROS scavenging enzymatic capacity, and activity of antioxidant enzymes CAT, APX, GPOX, GPX, and GR, and inhibited the activity of ROS producing enzymes such as GOX and NOX. Another mechanistic effect of NP consisted of the modulation of redox enzymes Trx, NTR, Fd, and FNR evolved in cellular homeostasis and maintaining reduced status in cells. Taken together, ZnO NP triggered more significant metabolic regulations allowing to mitigate the oxidative damage caused by Cd.