背景:据报道,印度不同州有几例寨卡病毒病的散发性病例和暴发。
目的:本文探讨了寨卡病毒(ZIKV)在印度中部博帕尔地区持续传播的可能性,这种疾病的最后一次爆发是在2018年。
方法:我们筛选了一组75例发热患者,这些患者的局部地方性发热原因如登革热检测呈阴性,基孔肯雅,肠热,疟疾,斑疹伤寒和两组无症状健康个体,以献血者(n=75)和产前母亲(n=75)为代表。我们使用实时聚合酶链反应(PCR)测试了发热患者的血液样本中的ZIKVRNA,对于健康的人来说,我们使用酶联免疫吸附试验测定了抗寨卡免疫球蛋白G(IgG)抗体。
结果:在通过实时PCR测定法测试的75个样品中的任何一个中均未检测到ZIKVRNA。在自愿献血者和产前母亲中,共发现10例(15.38%)和5例(6.66%)抗ZIKVIgG抗体血清阳性,分别。与血清阴性个体26.60(±5.12)岁相比,血清阳性组的年龄更高33.06(±10.83)岁(P=0.037)。
结论:这项研究,这是印度抗寨卡抗体血清阳性率的首次调查,报告健康人群中抗寨卡抗体的总血清阳性率为10%,暗示一个正在进行的,低水平,ZIKV在当地社区的无声传播。
BACKGROUND: Several sporadic cases and outbreaks of Zika virus disease have been reported from different states of India.
OBJECTIVE: This paper explored the possibility of any ongoing transmission of Zika virus (ZIKV) in the Bhopal region of Central India, where the last outbreak of this disease was reported in 2018.
METHODS: We screened a group of 75 febrile patients who had already tested negative for the locally endemic causes of fever like dengue, chikungunya, enteric fever, malaria, and scrub typhus and two groups of asymptomatic healthy individuals represented by blood donors (n = 75) and antenatal mothers (n = 75). We tested blood samples of febrile patients for ZIKV RNA using real-time polymerase chain reaction (PCR), and for the healthy individuals, we determined anti-zika immunoglobulin G (IgG) antibodies using enzyme-linked immunosorbent assay.
RESULTS: ZIKV RNA was not detected in any of the 75 samples tested by real-time PCR assay. Among the voluntary blood donors and antenatal mothers, a total of 10 (15.38%) and 5 (6.66%) individuals were found to be seropositive for anti-ZIKV IgG antibodies, respectively. The seropositive group was found to have higher age 33.06 (±10.83) years as compared to seronegative individuals 26.60 (±5.12) years (P = 0.037).
CONCLUSIONS: This study, which is the first survey of seroprevalence of anti-Zika antibodies from India, reports an overall seropositivity rate of 10% for anti-Zika antibodies among the healthy population, suggesting an ongoing, low level, silent transmission of ZIKV in the local community.