新的抗癌策略通过提高癌细胞的致死能力来减少对健康组织的副作用。开发具有良好生物利用度和选择性的有效颗粒仍然存在问题。对于不良特征,绿色化学被用来合成最好的化合物,或天然颗粒得到改善。光动力疗法(PDT),以酞菁(PC)为模型,仍然提供第二代敏化剂,它们补充有金属离子,如Zn2+,Al3+,或Ga3+。氢氧化八羧基酞菁镓(Ga(OH)PcOC),是为治疗皮肤癌而设计的,并被用作正常皮肤细胞系的促凋亡和促氧化剂,成纤维细胞(NHDF),和角质形成细胞(HaCaT),在体外具有对黑色素瘤癌细胞(Me45)的有希望的选择性。与以前报道的发现相比,ZnPcOC以较高的剂量作用于皮肤细胞系,对Ga(OH)PcOC的敏感性允许敏化剂剂量的有效降低。有效剂量,对于一种新型Ga(OH)PcOC颗粒,在Me45癌细胞上从30µM显着降低到6µM,使用24小时MTT活力测试,以及细胞计数促氧化和促凋亡测定。在没有活性氧(ROS)升高或凋亡诱导的情况下,有前途的光敏剂不会降低正常成纤维细胞和角质形成细胞的活力。对先前发现的改进是基于Ga的光敏剂对癌症Me45细胞的选择性更好,然后在Zn基化合物中观察到。
Novel anticancer strategies reduce side effects on healthy tissues by elevating the lethal abilities of cancer cells. The development of effective particles with good bioavailability and selectivity remains problematic. For undesirable features, green chemistry is used to synthesize the best compounds, or natural-based particles are improved. Photodynamic therapy (PDT), modelled on phthalocyanines (Pcs), still delivers second-generation sensitizers which are complemented with metal ions, such as Zn2+, Al3+, or Ga3+. Gallium octacarboxyphthalocyanine hydroxide (Ga(OH)PcOC), was designed for skin cancer treatment, and was used as a pro-apoptotic and pro-oxidative agent on normal skin cell lines, fibroblasts (NHDF), and keratinocytes (HaCaT), with promising selectivity against melanoma cancer cells (Me45) in vitro. Compared to the previous reported findings, where the ZnPcOC acted on the skin cell lines at higher doses, the sensitivities to the Ga(OH)PcOC allows for an effective reduction of the sensitizer dose. The effective dose, for a novel Ga(OH)PcOC particle, was significantly reduced from 30 µM to 6 µM on Me45 cancer cells, tested using 24 h MTT viability, as well as cytometric pro-oxidative and pro-apoptotic assays. The promising photosensitizer did not reduce viability in normal fibroblasts and keratinocytes without reactive oxygen species (ROS) elevation or apoptosis induction. The improvement to the previous findings is better Ga-based photosensitizer selectivity against the cancer Me45 cells, then observed in Zn-based compounds.