UNASSIGNED: Fungal diseases are frequently associated with elevated mortality rates in elasmobranchs. Currently, there is a notable absence of scientifically validated therapeutic medications that can ensure both effectiveness and safety when administered to this group of animals. The empirical prescription of azole antifungal agents, particularly voriconazole, has been posited as a potentially efficacious treatment approach for addressing most common mycoses in sharks and rays. However, there are still no published pharmacokinetic studies supporting its use in elasmobranchs and there is a lack of scientific base for its utilization in elasmobranchs.
UNASSIGNED: For this study, voriconazole was administered intravenously (IV) and intramuscularly (IM), at a single dose of 4 mg/kg to six adult undulate skates (Raja undulata). A washout period of 8 weeks was left between each route of administration. Blood samples were collected both before and at ten predetermined intervals after each dosing (0.25, 0.5, 1, 1.5, 2, 4, 8, 12, 24, and 36 h after drug administration). Plasma concentrations were quantified using a validated high-performance liquid chromatography method, and pharmacokinetic (PK) data was analyzed through non-compartmental methods.
UNASSIGNED: The mean extrapolated concentration at 0 h (C0) after IV administration was 27.19 ± 7.15 μg/mL and the mean peak plasma concentrations (Cmax) ± SEM after IM administration resulted 2.98 ± 0.28 μg/mL at a mean time to maximum concentration (T max) of 1.33 ± 0.17 h. Terminal half-lives were calculated and resulted 11.18 ± 1.32 h for IV injections and 9.59 ± 1.38 h for IM injections. The area under the curve extrapolated to infinity was determined as 58.14 ± 2.79 h·μg/ml following IV injections and 37.60 ± 6.67 h·μg/ml following IM injections. The IM-administered voriconazole exhibited a mean absolute bioavailability of 64.67 ± 11.47%.
UNASSIGNED: These discoveries provide backing for the possible application of voriconazole through the intramuscular route in undulate skates and support using lower dosage regimens compared to those required for oral administration, emphasizing the importance of conducting further pharmacokinetic studies with antifungals in elasmobranchs.

Until relatively recently commercial fisheries have been considered the main driving factor for elasmobranch population declines. However, this belief has begun to shift with the realization that recreational elasmobranch catches may equal or exceed commercial catches in some regions. Many recreational angling fisheries for elasmobranchs involve high participation in catch-and-release angling practices. However, high release rates may not necessarily equate to high survival rates. Therefore, to assist accurate assessment of the potential impact of recreational angling on elasmobranchs, we attempted to summarize and integrate currently available information on specific risk factors associated with recreational angling, alongside associated mortality rates, as well as information on angler behaviour as it relates to identified risk factors. We categorized the major angling-related effects into two groups: injury-induced effects; and biochemical disruption-induced effects; providing a summary of each group and outlining the main lethal and sub-lethal outcomes stemming from these. These outcomes include immediate and delayed post-release mortality, behavioural recovery periods (which may in-turn confer increased predation risks), chronic health impacts and capture-induced parturition and abortion. Additionally, we detailed a range of angling practices and equipment, including hook-type, hook removal and emersion (i.e. air exposure), as well as inter- and intra-specific factors, including aerobic scope, respiratory mode, body size and species-specific behaviours, which are likely to influence injury and/or mortality rates and should therefore be considered when assessing angling-related impacts. We then utilized these data to provide a range of actionable recommendations for both anglers and policymakers which would serve to reduce the population-level impact of recreational angling on these enigmatic animals.

The bowmouth guitarfish (Rhina ancylostomus) is among the most endangered of marine vertebrates, and evidence of severe declines and localized extinctions has been reported. Yet its life history and ecology suffer from a lack of scientific attention due to the scarcity and unpredictable movements of the species. By collecting opportunistic records from 2015 to 2023 during diving activities, this study describes for the first time the occurrence of a predictable aggregation of R. ancylostomus in the Gulf of Tadjoura (Djibouti). These data provide a key record of this species in the area, whose presence is strongly associated with sandy seabeds and corals at approximately 35 m depth. Based on the opportunistic sightings of R. ancylostomus during diving activity, Ras Eiro and Ras Korali appear to be the currently known core habitats for this species and may serve as breeding or feeding grounds. Overall, our data show that the Gulf of Tadjoura is a globally important conservation hotspot, and therefore its protection should be prioritized.

Until a few decades ago, play was considered a behavior unique to birds and mammals. Although play in other vertebrates is still a neglected research subject, data on it has been slowly accumulating, and are reviewed here. Now we know that animals as diverse as stingrays, cichlid fishes, monitor lizards, softshell turtles, and crocodiles can be unexpectedly playful. This knowledge has implications to broader theoretical problems, but much more research attention is needed to utilize its potential.

Elasmobranchs (sharks and rays) are charismatic cartilaginous fish, popular in public aquaria. Almost 200 shark and ray species are listed as threatened by the International Union for Conservation of Nature (IUCN), demonstrating the importance of captive breeding and research programmes. Limited studies investigate diseases of elasmobranchs in captive and free-living environments, and among available literature neoplasia is rarely reported, with even fewer cases of lymphoid neoplasia documented. This article outlines the first reports of lymphoid neoplasia in three elasmobranch species in which haematopoietic neoplasms have not been reported to date. It summarizes signalment, history and histopathologic findings in an undulate ray, Raja undulata (Lacepede), a common smooth-hound, Mustelus mustelus (Linnaeus) and a bat ray, Myliobatis californica (Gill). Lesions were confirmed in a wide range of tissues and evidence of lymphoid leukaemia was seen in two cases. This small-scale review demonstrates that lymphoid neoplasia should be considered as a differential diagnosis in elasmobranchs presenting with lethargy and anorexia and highlights the challenges of immunohistochemical work up.

The unsustainable trade in elasmobranch products, particularly fins, contributes to the decline of elasmobranch populations worldwide. Designing and implementing context-appropriate solutions to mitigate unsustainable trade requires a thorough analysis of markets. Here we assess the market component of the elasmobranch fin trade in the Bay of Bengal, Bangladesh, using a framework designed to analyse wildlife markets. Using a mixed-method approach, we characterised the market to identify the components contributing to unsustainable practices. By-catch retention levels were high leading to the development of a solid market. Trade on fins was prevalent due to a high price, lack of awareness, actors\' limited ability to adhere to regulations, and no strategies and incentives to limit fishing mortality. An imbalanced power and financial structure between actors were revealed, with some actors accessing unequal benefits from the market. Impediments for adopting conservation measures by low-access actors (e.g., fishers) with limited decision-making power or resources were evident. We also identified challenges to enforcement primarily due to limited reporting and issues identifying species and products. Fishers noted several socio-ecological, technical, and enforcement issues (e.g., policing instead of meaningful monitoring, punitive measures without facilitating compliance), that will require adequate time and resources to change practices. Lack of opportunities and information to adhere to regulations and increased enforcement has led to conflicts, non-compliance and unwillingness to report catches. The study has significantly strengthened the current understanding of Bangladesh\'s complex elasmobranch product market while highlighting critical knowledge gaps that must be addressed to inform and improve management decisions. Based on the findings, we recommend targeted actions to respond to the current market for mitigating elasmobranch product trade and moving towards establishing sustainable and ethical trade. Our work has both regional and global significance, given the role of the Bay of Bengal nations in the worldwide elasmobranch product market.

450 million years of evolution have given chondrichthyans (sharks, rays and allies) ample time to adapt perfectly to their respective everyday life challenges and cognitive abilities have played an important part in that process. The diversity of niches that sharks and rays occupy corresponds to matching diversity in brains and behaviour, but we have only scratched the surface in terms of investigating cognition in this important group of animals. The handful of species that have been cognitively assessed in some detail over the last decade have provided enough data to safely conclude that sharks and rays are cognitively on par with most other vertebrates, including mammals and birds. Experiments in the lab as well as in the wild pose their own unique challenges, mainly due to the handling and maintenance of these animals as well as controlling environmental conditions and elimination of confounding factors. Nonetheless, significant advancements have been obtained in the fields of spatial and social cognition, discrimination learning, memory retention as well as several others. Most studies have focused on behaviour and the underlying neural substrates involved in cognitive information processing are still largely unknown. Our understanding of shark cognition has multiple practical benefits for welfare and conservation management but there are obvious gaps in our knowledge. Like most marine animals, sharks and rays face multiple threats. The effects of climate change, pollution and resulting ecosystem changes on the cognitive abilities of sharks and stingrays remain poorly investigated and we can only speculate what the likely impacts might be based on research on bony fishes. Lastly, sharks still suffer from their bad reputation as mindless killers and are heavily targeted by commercial fishing operations for their fins. This public relations issue clouds people\'s expectations of shark intelligence and is a serious impediment to their conservation. In the light of the fascinating results presented here, it seems obvious that the general perception of sharks and rays as well as their status as sentient, cognitive animals, needs to be urgently revisited.

Coxalgia is one of the most common painful complaints in orthopedic surgery. The management of a \"traumatic\" hip is relatively well codified, however atraumatic coxalgia is more difficult to diagnose and explore. The history and a well-conducted clinical examination are essential for the management of coxalgia. The diagnosis is already well oriented by a simple X-ray and the presence or absence of inflammatory signs on the blood test. This article aims to provide a guideline for the diagnostic approach to coxalgia.
La coxalgie est une des plaintes douloureuses les plus fréquentes en chirurgie orthopédique. La prise en charge d’une hanche «traumatique» est relativement bien codifiée, par contre les coxalgies atraumatiques sont plus difficiles à diagnostiquer et explorer. L’anamnèse et un examen clinique bien conduit sont primordiaux pour la prise en charge de la coxalgie. Le diagnostic est déjà bien orienté par une simple radiographie et la présence ou non de signe inflammatoire à la prise de sang. Cet article vise à donner une ligne de conduite à la démarche diagnostique face à la coxalgie.

The taxon Elasmobranchii (sharks and rays) contains one of the long-established evolutionary lineages of vertebrates with a tantalizing collection of species occupying critical aquatic habitats. To overcome the current limitation in molecular resources, we launched the Squalomix Consortium in 2020 to promote a genome-wide array of molecular approaches, specifically targeting shark and ray species. Among the various bottlenecks in working with elasmobranchs are their elusiveness and low fecundity as well as the large and highly repetitive genomes. Their peculiar body fluid composition has also hindered the establishment of methods to perform routine cell culturing required for their karyotyping. In the Squalomix consortium, these obstacles are expected to be solved through a combination of in-house cytological techniques including karyotyping of cultured cells, chromatin preparation for Hi-C data acquisition, and high fidelity long-read sequencing. The resources and products obtained in this consortium, including genome and transcriptome sequences, a genome browser powered by JBrowse2 to visualize sequence alignments, and comprehensive matrices of gene expression profiles for selected species are accessible through https://github.com/Squalomix/info.

The development of third-generation sequencing technology has brought significant changes and influences on genomics. Compared to the second-generation sequencing methods, the third-generation technologies produce around 100 times longer reads to reveal new genomic variations that complete long-term gaps in the human reference genome. However, these reads\' excessive length and high error rate severely increase the amount of data and alignment cost. The traditional data analysis platform and serial sequence alignment method can not effectively deal with large-scale long read alignment. There is a critical need for a novel data analysis platform that can deliver fast alignment of large-scale sequences to solve the problem of long read alignment. High-performance computing platforms and efficient, scalable algorithms based on these platforms have significant potential to impact sequence analysis approaches. This paper presented minimapR, a multi-level parallel long-read alignment tool based on minimap2, a popular third-generation read aligner. MinimapR is developed based on the new high-performance distributed framework Ray. Ray fully integrates with the Python environment and can be easily installed with pip. MinimapR can utilize the power of multiple computing nodes, significantly accelerating alignment speeds without sacrificing sensitivity. The minimapR tool was tested on 64 nodes and demonstrated a 50 fold increase in speed with 78 % parallel efficiency. The source code and user manual of minimapR are freely available at https://github.com/Geehome/minimapR.