约90%的糖尿病男性有不同程度的睾丸功能障碍。本研究调查了雷诺嗪对T1DM诱导的大鼠睾丸功能障碍的可能有益后果。将32只雄性SD大鼠分为4组;正常,糖尿病(单一50毫克/千克STZ,I.P.)和雷诺嗪(40和80mg/kg,口头)。本研究表明,雷诺嗪的降血糖作用显着改善了最终大鼠的睾丸重量和体重,以及血液中睾酮的浓度,精子计数,和生存能力,所有这些都与STZ诱导的睾丸功能障碍有关。此外,正如还原型谷胱甘肽(GSH)活性升高和丙二醛(MDA)水平降低所证明的那样,给予雷诺嗪的糖尿病大鼠显示出氧化剂/抗氧化剂比例的显着改善。此外,当雷诺嗪给药时,Beclin-1浓度显著升高,同时硫氧还蛋白相互作用蛋白(TXNIP)和白细胞介素-18(IL-18)浓度显著降低.尽管雷诺嗪表现出炎症减少,如睾丸中核因子-κB(NF-κB)和分化簇(CD68)的较低表达所见,这些生化结果通过胰腺和睾丸组织的形态学和组织病理学结果的改善得到验证.总之,每日口服雷诺嗪(40和80mg/kg),持续8周,通过其剂量依赖性抗氧化和抗炎作用,可能是治疗T1DM诱导的睾丸功能障碍的一种有前景的疗法.
Approximately 90% of diabetic males have varying degrees of testicular dysfunction. The current study investigates the possible beneficial consequences of
ranolazine against T1DM-induced testicular dysfunction in rats. Thirty-two male Sprague Dawley rats were assorted into 4 groups; normal, diabetic (single 50 mg/kg STZ, I.P.) and
ranolazine (40 and 80 mg/kg, orally). The present investigation revealed that the hypoglycemic impact of
ranolazine significantly improved the testicular weight and body weight of the final rats, as well as the concentration of blood testosterone, sperm count, and viability, all of which were associated with STZ-induced testicular dysfunction. Furthermore, as demonstrated by elevated reduced glutathione (GSH) activity and lowered malondialdehyde (MDA) levels, diabetic rats administered ranolazine showed a noteworthy improvement in the oxidant/antioxidant ratio. Furthermore, a substantial rise in beclin-1 concentration was seen in conjunction with a significant decrease in thioredoxin-interacting protein (TXNIP) and interleukin-18 (IL-18) concentrations when ranolazine was administered. Although
ranolazine exhibited a reduction in inflammation as seen by lower expression of nuclear factor-κB (NF-κB) and cluster of differentiation (CD68) in the testicles, these biochemical findings were validated by improvements in the morphological and histopathological outcomes of both the pancreatic and testicular tissues. In conclusion, daily oral administration of
ranolazine (40 and 80 mg/kg) for 8 weeks could be a promising therapy for T1DM-induced testicular dysfunction through its dose-dependent anti-oxidant and anti-inflammatory effects.