■放射治疗是肺癌的有效局部治疗方法。然而,基因和放射治疗之间的相互作用是多方面和复杂的。因此,我们探讨了miR-93-5p在增殖中的作用,凋亡,和A549细胞的迁移能力。同时,我们还研究了miR-93-5p与电离辐射(IR)之间的相互作用.
■细胞计数套件-8,transwell,并进行凋亡测定以测量增殖,迁移,和凋亡能力。使用starBasev3.0预测miR-93-5p及其靶基因在肺癌中的表达水平。然后,数据使用qPCR和westernblot进行验证.
■miR-93-5p显著促进A549细胞增殖(P<0.01)和迁移能力(P<0.001)。GasderminE(GSDME)被鉴定为miR-93-5p的推定靶标,并且与miR-93-5p呈负相关(P<0.001)。miR-93-5p过表达显著降低A549中GSDME(P<0.001)。有趣的是,miR-93-5p降低IR后A549细胞增殖(P<0.01)和细胞迁移(P<0.01),增加细胞凋亡(P<0.01)。
■miR-93-5p被认为通过增强A549细胞增殖和迁移在肺癌中发挥致癌作用。它可以增强红外条件下放疗的敏感性。我们推测miR-93-5p/GSDME通路受到抑制,当细胞暴露于IR时,激活GSDME相关的焦亡途径。因此,miR-93-5p可以克服放疗抵抗,提高放疗疗效。
UNASSIGNED: Radiation therapy is an effective local therapy for lung cancer. However, the interaction between genes and radiotherapy is multifaceted and intricate. Therefore, we explored the role of miR-93-5p in the proliferation, apoptosis, and migration abilities of A549 cells. Simultaneously, we also investigated the interactions between miR-93-5p and ionizing radiation (IR).
UNASSIGNED: Cell Counting Kit-8, transwell, and apoptotic assay were performed to measure the proliferation, migration, and apoptosis abilities. The expression levels of miR-93-5p and its target gene in lung cancer were predicted using starBase v3.0. Then, data were validated using qPCR and western blot.
UNASSIGNED: miR-93-5p significantly promoted the proliferation (P < 0.01) and migration abilities (P < 0.001) of A549 cells. Gasdermin E (GSDME) was identified to be a putative target of miR-93-5p and had a negative correlation with miR-93-5p (P < 0.001). Overexpression of miR-93-5p significantly decreased GSDME in A549 (P < 0.001). Interestingly, miR-93-5p decreased cell proliferation (P < 0.01) and cell migration (P < 0.01) and increased apoptosis (P < 0.01) in A549 cells after exposure to IR.
UNASSIGNED: miR-93-5p is presumed to play an oncogenic role in lung cancer by enhancing A549 cell proliferation and migration. It can enhance the sensitivity of radiotherapy under IR conditions. We speculate that the miR-93-5p/GSDME pathway was inhibited, activating the GSDME-related pyroptosis pathway when the cells were exposed to IR. Therefore, miR-93-5p can overcome resistance to radiotherapy and improve the efficacy of radiotherapy.