Vigna属(Leguminosae)包括约150个物种,分为五个亚属。本研究旨在提高对Vigna核型多样性和进化的认识,通过不同的细胞遗传学和DNA含量方法使用新的和以前发表的数据。在维格纳亚属中,我们观察到rDNA模式的随机分布。35SrDNA的位置不同,从终端到近端,在数量上,范围从一(V.针叶,五、subg。Ceratotropis)到七对(V.unguiculata亚种。unguiculata,五、subg。维格纳)。另一方面,5SrDNA的数量是保守的(一对或两对),除了V.radiata(V.subg.Ceratotropis),有三双。基因组大小在属内相对保守,V.oblongifolia和V.unguiculata亚种的1C=0.43至0.70pg。unguiculata,分别,都属于V.subg。维格纳.然而,我们观察到DNA含量与35SrDNA位点的数量之间呈正相关。此外,来自染色体特异性BAC-FISH的数据表明,祖先的35SrDNA基因座在Vigna内的6号染色体上保守。考虑到rDNA位点数量和位置的快速多样化,这种保护是令人惊讶的,并表明,更多的网站可能已经从这个祖先的所在地扩散。
The genus Vigna (Leguminosae) comprises about 150 species grouped into five subgenera. The present study aimed to improve the understanding of karyotype diversity and evolution in Vigna, using new and previously published data through different cytogenetic and DNA content approaches. In the Vigna subgenera, we observed a random distribution of rDNA patterns. The 35S rDNA varied in position, from terminal to proximal, and in number, ranging from one (V. aconitifolia, V. subg. Ceratotropis) to seven pairs (V. unguiculata subsp. unguiculata, V. subg. Vigna). On the other hand, the number of 5S rDNA was conserved (one or two pairs), except for V. radiata (V. subg. Ceratotropis), which had three pairs. Genome size was relatively conserved within the genus, ranging from 1C = 0.43 to 0.70 pg in V. oblongifolia and V. unguiculata subsp. unguiculata, respectively, both belonging to V. subg. Vigna. However, we observed a positive correlation between DNA content and the number of 35S rDNA sites. In addition, data from chromosome-specific BAC-FISH suggest that the ancestral 35S rDNA locus is conserved on chromosome 6 within Vigna. Considering the rapid diversification in the number and position of rDNA sites, such conservation is surprising and suggests that additional sites may have spread out from this ancestral locus.