■提出了一种原型红外衰减全反射(IR-ATR)激光光谱系统,该系统设计用于在关节镜手术期间根据人体软骨组织的组织学健康状况对其进行体内分类。在真实世界的体内应用之前,这种所谓的骨关节炎(OA)扫描仪已经在体外条件下进行了测试,揭示了与复杂样品基质和相应获得的稀疏光谱数据集相关的挑战.
■在不同接触压力(即,0.2-0.5MPa)允许记录与具有高时间分辨率的体内条件相当的软骨退化特征IR特征。之后,软骨样本基于临床公认的骨关节炎软骨组织病理学评估(OARSI)系统进行评估,并与获得的稀疏IR数据进行关联.
■在获得的稀疏IR数据和用于稀疏偏最小二乘判别分析(SPLSDA)以识别与软骨状况相关的光谱区域的先前测量的FTIR数据之间,观察到酰胺和碳水化合物信号行为几乎相同。0.3和0.4MPa之间的接触压力似乎为圆柱形(d=3mm)探针尖端提供了最佳的稀疏红外光谱。
■激光辐照IR-ATR光谱是一种有前途的分析技术,可用于未来关节镜应用以区分健康和骨关节炎软骨组织。然而,这项研究还表明,基于激光的分析仪和通过红外透明光纤电缆的关节镜ATR探头之间的灵活连接可能会影响获得的红外数据的鲁棒性,需要进一步改进。
UNASSIGNED: A prototype infrared attenuated total reflection (IR-ATR) laser spectroscopic system designed for in vivo classification of human cartilage tissue according to its histological health status during arthroscopic surgery is presented. Prior to real-world in vivo applications, this so-called osteoarthritis (OA) scanner has been tested at in vitro conditions revealing the challenges associated with complex sample matrices and the accordingly obtained sparse spectral datasets.
UNASSIGNED: In vitro studies on human knee cartilage samples at different contact pressures (i.e., 0.2-0.5 MPa) allowed recording cartilage degeneration characteristic IR signatures comparable to in vivo conditions with high temporal resolution. Afterwards, the cartilage samples were assessed based on the clinically acknowledged osteoarthritis cartilage histopathology assessment (OARSI) system and correlated with the obtained sparse IR data.
UNASSIGNED: Amide and carbohydrate signal behavior was observed to be almost identical between the obtained sparse IR data and previously measured FTIR data used for sparse partial least squares discriminant analysis (SPLSDA) to identify the spectral regions relevant to cartilage condition. Contact pressures between 0.3 and 0.4 MPa seem to provide the best sparse IR spectra for cylindrical (d = 3 mm) probe tips.
UNASSIGNED: Laser-irradiating IR-ATR spectroscopy is a promising analytical technique for future arthroscopic applications to differentiate healthy and osteoarthritic cartilage tissue. However, this study also revealed that the flexible connection between the laser-based analyzer and the arthroscopic ATR-probe via IR-transparent fiberoptic cables may affect the robustness of the obtained IR data and requires further improvements.