背景:这项研究的目的是比较不同的A整合素和金属蛋白酶与血小板反应蛋白基序的水平(ADAMTS;即,ADAMTS-1,-4和-9])与抑制健康牙髓和症状性不可逆牙髓炎细胞外基质中ADAMTS的蛋白聚糖和金属蛋白酶3组织抑制剂(TIMP-3)水平的分解有关。
方法:收集48名参与者诊断为健康和有症状的不可逆牙髓炎的牙髓组织样本。健康和发炎的纸浆直接转移到Eppendorf管中(Labosel,伊斯坦布尔,土耳其)并储存在-80°C(Nüve-DF490;Nüve,安卡拉,土耳其),直至进一步使用。对牙齿进行常规根管治疗,之后,治疗过程完成。使用酶联免疫吸附测定试剂盒(USCN,武汉,中国)。参数数据采用独立样本t检验和单向方差分析,Kruskal-Wallis和Mann-WhitneyU检验用于非参数数据。
结果:健康牙髓组和牙髓炎组的ADAMTS-1和-9和TIMP-3水平之间存在统计学上的显着差异(P<0.05),但ADAMTS-4水平无显著差异(P>.05)。结果发现,与健康牙髓组相比,牙髓炎组的ADAMTS-1和-9水平更高,而TIMP-3水平降低(P<0.05)。
结论:作为这项研究的结果,结论ADAMTS-1和-9和TIMP-3可能在牙髓炎症中起作用,而ADAMTS-4与牙髓炎症无关。
BACKGROUND: The aim of this study was to compare the levels of different A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS; ie, ADAMTS-1, -4, and -9]) related to the breakdown of the proteoglycans and tissue inhibitor of metalloproteinase 3 (TIMP-3) levels that inhibit ADAMTS in the extracellular matrix of healthy pulp and symptomatic irreversible pulpitis.
METHODS: Pulp tissue samples diagnosed with healthy and symptomatic irreversible pulpitis were collected from 48 participants. Healthy and inflamed pulps were directly transferred to Eppendorf tubes (Labosel, Istanbul, Turkey) and stored at -80°C (Nüve-DF490; Nüve, Ankara, Turkey) until further use. Routine root canal treatment procedures for the teeth were performed, after which the treatment process was completed. The levels of ADAMTS-1, -4, and -9 and TIMP-3 were measured in supernatants of human dental pulp tissue extracts using enzyme-linked immunosorbent assay kits (USCN, Wuhan, China). The independent sample t test and 1-way analysis of variance were used for parametric data, and the Kruskal-Wallis and Mann-Whitney U tests were used for nonparametric data.
RESULTS: There was a statistically significant difference between ADAMTS-1 and -9 and TIMP-3 levels of the healthy pulp and pulpitis groups (P < .05), but there was no significant difference for ADAMTS-4 levels (P > .05). It was found that the levels of ADAMTS-1 and -9 were higher in the pulpitis group compared with the healthy pulp group, whereas the TIMP-3 level decreased (P < .05).
CONCLUSIONS: As a result of this study, it was concluded that ADAMTS-1 and -9 and TIMP-3 might have a role in pulpal inflammation, whereas ADAMTS-4 was not related to pulpal inflammation.