■牛皮癣是一种广泛的慢性疾病,经常复发的免疫介导的皮肤病,并且对患者的身心健康极为有害,给整个医疗保健系统带来巨大的痛苦和巨大的经济负担。在十多年的临床应用中,银屑灵(PSORI-CM01)的优化配方一直证明其治疗银屑病的有效性。然而,其潜在机制在很大程度上仍未被探索。
■进行网络药理学分析以预测PSORI-CM01治疗银屑病的机制和保护作用。随后,我们收集了21名银屑病患者的血液样本,双盲,和microRNA表达谱的双重虚拟临床试验。最后,实验证实PSORI-CM01通过调节miR-20a-3p和miR-3184-3p表达改善银屑病。
■作为网络药理学分析的结果,PSORI-CM01通过调节自噬改善银屑病,细胞凋亡,细胞增殖,和抗炎过程。在靶miRNA调控网络中,这些关键靶标主要与hsa-miR-20a-3p的调控有关,hsa-miR-155-5p,has-miR-3184-3p,hsa-miR-328-3p和hsa-miR-124-3p。根据microRNA表达谱的结果,与健康对照组相比,PSORI-CM01治疗组表现出5个上调基因和16个下调基因.特别是,miR-20a-3p和miR-3184-3p是主要差异表达的microRNAs,它们在涉及自噬的信号通路中显著富集,凋亡,扩散,和抗炎。进一步的实验证实,PSORI-CM01有效调节miR-20a-3p和miR-3184-3p,导致自噬增加。
■我们通过结合网络药理学和PBMC中miRNA表达谱的临床研究证明,PSORI-CM01有效调节miR-20a-3p和miR-3184-3p,导致自噬增加和角质形成细胞增殖减少。
UNASSIGNED: Psoriasis is a widespread chronic, immune-mediated skin disease with frequent recurrences, and is extremely harmful to the physical and mental health of patients, causing enormous suffering and exerting considerable economic burdens on the health care system as a whole. In more than a decade of clinical use, the optimized formula of Yinxieling (PSORI-CM01) has consistently demonstrated its effectiveness for treating psoriasis. However, its underlying mechanism remains largely unexplored.
UNASSIGNED: The network pharmacology analysis was conducted to predict the mechanism and protective effect of PSORI-CM01 in treating psoriasis. Subsequently, we collected blood samples from 21 patients with psoriasis as part of a randomized, double-blind, and double-dummy clinical trial for microRNA expression profiling. Finally, it was experimentally confirmed that PSORI-CM01 improved psoriasis by regulating miR-20a-3p and miR-3184-3p expression.
UNASSIGNED: As a result of the network pharmacology analysis, PSORI-CM01 improved psoriasis through the regulation of autophagy, cellular apoptosis, cellular proliferation, and anti-inflammatory processes. In the target-miRNA regulatory network, these key targets were mainly associated with the regulation of hsa-miR-20a-3p, hsa-miR-155-5p, has-miR-3184-3p, hsa-miR-328-3p and hsa-miR-124-3p. Based on the microRNA expression profiling results, the PSORI-CM01 treatment group exhibited five up-regulated genes and 16 down-regulated genes compared with the healthy control group. In particular, miR-20a-3p and miR-3184-3p were the primary differentially expressed microRNAs, and they were significantly enriched in the signaling pathways involving autophagy, apoptosis, proliferation, and anti-inflammation. Further experiments confirmed that PSORI-CM01 effectively regulates miR-20a-3p and miR-3184-3p, resulting in increased autophagy.
UNASSIGNED: We demonstrated by combining network pharmacology and clinical studies of miRNA expression profiles in PBMCs that PSORI-CM01 effectively modulated miR-20a-3p and miR-3184-3p, leading to an increase in autophagy and a decrease in keratinocyte proliferation.