这项研究评估了用维生素B6治疗母犬假孕是否可以调节孕激素(PR)受体的子宫表达,雌激素(ERα),雄激素(AR),甲状腺激素(TRα)和kisspeptin/Kiss1r系统。在接受安慰剂(n=6);卡麦角林(5μg/kg/天;n=6)或维生素B6(50mg/kg/天;n=6)的组中,对18只假孕母犬进行了20天的治疗。在给药的第1天和120小时后收集血液以测量血清催乳素(PRL)。治疗后,他们被卵巢子宫切除和子宫碎片收集进行组织形态学和免疫组织化学评价PR,ERα,AR,TRα,Kiss1和Kiss1r.卡麦角林或维生素B6治疗120小时后,母狗的PRL水平降低了,证实这些药物的抗催乳素作用。此外,不管治疗,动物表现出与发情一致的子宫组织形态计量学。PR在所有区域均显示强免疫染色,并且在深层腺体中用维生素B6治疗的动物中观察到该受体的得分增加。相比之下,ERα和Kiss1R受体在所有子宫区域均显示弱至无免疫染色,组间无变化。关于AR,大多数接受维生素B6治疗的动物在深腺和子宫肌层标记评分方面呈现增加趋势.相比之下,在维生素B6和卡麦角林治疗中,与对照组相比,观察到TRα标记评分降低.此外,在子宫内膜表面,与假孕对照组相比,服用卡麦角林后Kiss1的标记区域减少。这些发现为使用维生素B6作为药物提供了宝贵的见解,其作用类似于卡麦角林,可减少母犬的PRL和子宫调节。
This study evaluated whether the treatment of pseudopregnancy in bitches with vitamin B6 modulates uterine expression of receptors for progesterone (PR), oestrogen (ERα), androgen (AR), thyroid hormone (TRα) and the kisspeptin/Kiss1r system. Eighteen pseudopregnant bitches were treated for 20 days in groups receiving placebo (n = 6); cabergoline (5 μg/kg/day; n = 6); or vitamin B6 (50 mg/kg/day; n = 6). Blood was collected on the 1st day of drug administration and 120 h later to measure serum prolactin (PRL). After treatment, they were ovariohysterectomized and uterine fragments were collected for histomorphometry and immunohistochemical evaluation of PR, ERα, AR, TRα, Kiss1 and Kiss1r. After 120 h of cabergoline or vitamin B6 treatment, PRL levels were reduced in the bitches, confirming the antiprolactinemic effect of these drugs. Furthermore, regardless of treatment, the animals exhibited uterine histomorphometry consistent with dioestrus. The PR showed strong immunostaining in all regions and an increase in scores was observed for this receptor in animals treated with vitamin B6 in deep glands. In contrast, ERα and Kiss1R receptors showed weak to no immunostaining in all uterine regions and no changes between groups. Regarding AR, most animals treated with vitamin B6 showed increased trends in the deep gland and myometrium marking scores. In contrast, in both vitamin B6 and cabergoline treatments, a reduction in TRα marking scores was observed compared to the control group. In addition, on the endometrial surface, a reduction was observed in the marked area of Kiss1 after administration of cabergoline when compared to the pseudopregnant control group. These findings shed valuable insight into the use of vitamin B6 as a drug with actions similar to cabergoline in reducing PRL and uterine modulation in bitches.