副淋病杆菌是一种果胶分解植物病原菌,对栽培植物造成很高的经济损失。这种植物病原体的高破坏性潜力来自植物细胞壁降解酶的有效生产,即,果胶酶,纤维素酶和蛋白酶,除了副毒力因素如运动性的影响,铁载体,生物膜和脂多糖(LPS)。LPS属于病原体相关分子模式(PAMPs),在植物定殖和与宿主防御系统的相互作用中起重要作用。因此,我们决定研究不同菌株的LPS的O-多糖(OPS)的异质性。寻找共享OPS分子相同结构的菌株的所选基因组和表型特征之间的关联。在目前的研究中,从1980年代在芬兰(SCC3193)或2013年在波兰(IFB5432)获得的两种parmentieri菌株的LPS中分离OPS。利用1D和2DNMR光谱(1H,DQF-COSY,托西,罗西,HSQC,HSQC-TOCSY和HMBC)以及化学方法。天然多糖的糖和甲基化分析,组成单糖的绝对构型分配和NMR光谱数据表明,在不同国家分离的这两个P.parmentieri菌株具有相同的OPS结构,具有非常罕见的残基5,7-二氨基-3,5,7,9-四脱氧-1-甘油-1-甘露聚糖-非-2-ulosonic酸(假氨基酸)在C-8位被取代:→3-G-P-1-3-先前的研究表明,其他三个parmentieri菌株,即IFB5427、IFB5408和IFB5443表现出与SCC3193和IFB5432不同的OPS分子。所进行的面向生物多样性的测定表明,具有相同OPS结构的ParmentieriIFB5427和IFB5408菌株产生了最高的全基因组相似性,根据平均核苷酸同一性分析,除了在研究的P.parmentieri菌株中浸渍菊苣组织的最大能力之外。目前的研究证明了一种新型的OPS结构,具有至少两种parmentieri菌株(SCC3193和IFB5432)的特征,并讨论了在广泛的基因组和表型相关背景下观察到的ParmentieriOPS的异质性。
Pectobacterium parmentieri is a pectinolytic plant pathogenic bacterium causing high economic losses of cultivated plants. The highly devastating potential of this phytopathogen results from the efficient production of plant cell wall-degrading enzymes, i.e., pectinases, cellulases and proteases, in addition to the impact of accessory virulence factors such as motility, siderophores, biofilm and lipopolysaccharide (LPS). LPS belongs to pathogen-associated molecular patterns (PAMPs) and plays an important role in plant colonization and interaction with the defense systems of the host. Therefore, we decided to investigate the heterogeneity of O-polysaccharides (OPS) of LPS of different strains of P. parmentieri, in search of an association between the selected genomic and phenotypic features of the strains that share an identical structure of the OPS molecule. In the current study, OPS were isolated from the LPS of two P. parmentieri strains obtained either in Finland in the 1980s (SCC3193) or in Poland in 2013 (IFB5432). The purified polysaccharides were analyzed by utilizing 1D and 2D NMR spectroscopy (1H, DQF-COSY, TOCSY, ROESY, HSQC, HSQC-TOCSY and HMBC) in addition to chemical methods. Sugar and methylation analyses of native polysaccharides, absolute configuration assignment of constituent monosaccharides and NMR spectroscopy data revealed that these two P. parmentieri strains isolated in different countries possess the same structure of OPS with a very rare residue of 5,7-diamino-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (pseudaminic acid) substituted in the position C-8: →3)-β-d-Galf-(1→3)-α-d-Galp-(1→8)-β-Pse4Ac5Ac7Ac-(2→6)-α-d-Glcp-(1→6)-β-d-Glcp-(1→. The previous study indicated that three other P. parmentieri strains, namely IFB5427, IFB5408 and IFB5443, exhibit a different OPS molecule than SCC3193 and IFB5432. The conducted biodiversity-oriented assays revealed that the P. parmentieri IFB5427 and IFB5408 strains possessing the same OPS structure yielded the highest genome-wide similarity, according to average nucleotide identity analyses, in addition to the greatest ability to macerate chicory tissue among the studied P. parmentieri strains. The current research demonstrated a novel OPS structure, characteristic of at least two P. parmentieri strains (SCC3193 and IFB5432), and discussed the observed heterogenicity in the OPS of P. parmentieri in a broad genomic and phenotype-related context.