腮腺炎是由腮腺炎病毒(MuV)引起的儿童常见感染。无菌性脑膜炎和脑炎是腮腺炎以及睾丸炎和卵巢炎的常见症状,可在男性和女性中出现,分别。我们已经使用计算工具:RNA22,miRanda和psRNATarget来预测microRNA-mRNA结合位点,以发现推定的microRNA在宿主对腮腺炎病毒感染的反应中起作用。我们的计算研究表明hsa-mir-3155a最有可能参与腮腺炎感染。通过预测hsa-mir-3155a与MuV基因组的结合位点进一步研究了这一点。此外,使用MC-Fold和MC-Sym进行结构预测,分别用于预测miRNA和mRNA的3D结构。通过分子对接模拟研究证实了miRNA-mRNA之间的相互作用谱。一起来看,已发现推定的miRNA(hsa_miR_6794_5p)最有可能参与MuV感染中转录活性的调节。
Mumps is a common childhood infection caused by the mumps virus (MuV). Aseptic meningitis and encephalitis are usual symptoms of mumps together with orchitis and oophoritis that can arise in males and females, respectively. We have used computational tools: RNA22, miRanda and
psRNATarget to predict the microRNA-mRNA binding sites to find the putative microRNAs playing role in the host response to mumps virus infection. Our computational studies indicate that hsa-mir-3155a is most likely involved in mumps infection. This was further investigated by the prediction of binding sites of hsa-mir-3155a to the MuV genome. Additionally, structure prediction using MC-Fold and MC-Sym, respectively has been applied to predict the 3D structures of miRNA and mRNA. The miRNA-mRNA interaction profile between has been confirmed through molecular docking simulation studies. Taken together, the putative miRNA (hsa_miR_6794_5p) has been found to be most likely involved in the regulation of transcriptional activity in the MuV infection.