农药在法医毒理学中起着重要作用,通常被归类为一类化学品。尽管他们普遍认为是团结的,杀虫剂包含一系列化合物,包括有机磷酸酯,氨基甲酸酯,拟除虫菊酯或有机氯,其中,每个都有不同程度的毒性。由于样品的复杂性和这些化合物的高毒性,死后样品中的农药分析可能是困难的。这项研究的目的是开发和验证一个易于使用的,敏感,和鲁棒的方法,使用超高效液相色谱-串联质谱法将其纳入常规流程,用于死后血液样品中的农药分析。本文描述的是一种流线型,迅速,然而高效的方法促进筛选,定性评估,和15种农药的定量确认,包括啶虫脒,氮杂磷乙基,bendiocarb,克百威,氯芬霉素,乐果,吡虫啉,马拉硫磷,甲硫威,灭多威,对硫磷,pirimicarb,马钱子碱,四氯乙烯磷,以及死后血液中的噻虫啉,认识到血液在法医调查中的关键作用。所开发的方法在10至200ng/mL范围内呈线性关系;检出限在1至10ng/mL之间,取决于化合物;成功评估了1-2、5和10的稀释比;在研究的时间间隔内,有8种物质显示出最大的稳定性。这种UHPLC-MS/MS方法在毒理学实验室中是有用和强大的工具,因为它是快速的,简单,有效,并且值得信赖。这一验证的结果突出了分析方法的稳健性,为准确和灵敏地检测死后血液中的农药提供了有价值的工具。准备好常规实施,这种方法已经在疑似中毒病例中获得成功,承诺提高法医农药分析的标准。
Pesticides play an important role in forensic toxicology and are usually classified as a single class of chemicals. Despite their commonly perceived unity, pesticides encompass a spectrum of compounds, including organophosphates, carbamates, pyrethroids or organochlorines, among others, each with varying degrees of toxicity. Pesticide analysis in post-mortem samples can be difficult due to the complexity of the samples and to the high toxicity of these compounds. The aim of this study was to develop and validate an easy to use, sensitive, and robust method, using ultra-performance liquid chromatography-tandem mass spectrometry to be incorporated in the routine flow for pesticide analysis in post-mortem blood samples. Described herein is a streamlined, expeditious, yet highly efficient method facilitating the screening, qualitative assessment, and quantitative confirmation of 15 pesticides, including acetamiprid, azinphos-ethyl, bendiocarb, carbofuran, chlorfenvinphos, dimethoate, imidaclopride, malathion, methiocarb, methomyl, parathion, pirimicarb, strychnine, tetrachlorvinphos, and thiacloprid in post-mortem blood, recognizing the pivotal role blood plays in forensic investigations. The developed method was linear from 10 to 200 ng/mL; limits of detection were between 1 and 10 ng/mL, depending on the compound; it was successfully evaluated a dilution ratio of 1-2, 5 and 10; and 8 substances showed maximum stability for the time interval studied. This UHPLC-MS/MS method is useful and a powerful tool in a toxicology lab because it is fast, simple, effective, and trustworthy. The results of this validation highlight the robustness of the analytical method, providing a valuable tool for the accurate and sensitive detection of pesticides in post-mortem blood. Poised for routine implementation, this method has already found success in suspected intoxication cases, promising to elevate the standards of forensic pesticide analysis.