假体周围感染是患者护理中的重要因素,也具有重要的经济意义。与生物膜感染相关的置换手术的数量正在增加,并将在接下来的几十年中继续这样做。为了减轻患者的健康负担和医疗保健部门的成本,抗这种感染的植入材料的新解决方案是必要的。本研究研究了钴铬钼(CoCrMo)基植入材料的不同表面修饰及其对生物膜发育的影响。三个光滑表面(CoCrMo,CoCrMoTiN,和CoCrMo抛光)和三个粗糙表面(CoCrMo多孔涂层,CoCrMocpTi,和CoCrMoTCP)进行了比较。假体周围感染中最常见的感染因子是金黄色葡萄球菌和凝固酶阴性葡萄球菌(例如,表皮葡萄球菌),因此,这两个物种的菌株已被选为模式生物。生物膜在材料盘上生长48小时,细胞数,多糖含量,测量生物膜的蛋白质含量。此外,参与早期生物膜发育的基因调控(S.金黄色葡萄球菌icaA,icaC,fnba,fnbB,clfB,atl;表皮葡萄球菌,aap)使用RT-q-PCR检测。将所有结果与没有改性的基础合金进行比较。结果表明,表面粗糙度与生物膜结构的蛋白质和多糖含量以及在不同表面修饰上生长的生物膜的基因表达之间存在相关性。这得到与粗糙和光滑表面类型相关的生物膜的显著不同的蛋白质和多糖含量的支持。此外,早期生物膜基因(特别是icaA,icaC,和aap)在48小时在粗糙表面上与对照相比在统计学上显着下调。CoCrMoTiN和抛光的CoCrMo是两种光滑的表面改性,在低生物膜含量的基础上表现最好。
Periprosthetic infections are an eminent factor in patient care and also having significant economic implications. The number of biofilm-infection related replacement surgeries is increasing and will continue to do so in the following decades. To reduce both the health burden of the patients and the costs to the healthcare sector, new solutions for implant materials resistant to such infections are necessary. This study researches different surface modifications of cobalt-chromium-molybdenum (CoCrMo) based implant materials and their influence on the development of biofilms. Three smooth surfaces (CoCrMo, CoCrMo TiN, and CoCrMo polished) and three rough surfaces (CoCrMo porous coated, CoCrMo cpTi, and CoCrMo TCP) are compared. The most common infectious agents in periprosthetic infections are Staphylococcus aureus and Coagulase-negative staphylococci (e.g., Staphylococcus epidermidis), therefore strains of these two species have been chosen as model organisms. Biofilms were grown on material disks for 48 h and cell number, polysaccharide content, and protein contend of the biofilms were measured. Additionally, regulation of genes involved in early biofilm development (S. aureus icaA, icaC, fnbA, fnbB, clfB, atl; S. epidermidis atlE, aap) was detected using RT-q-PCR. All results were compared to the base alloy without modifications. The results show a correlation between the surface roughness and the protein and polysaccharide content of biofilm structures and also the gene expression of the biofilms grown on the different surface modifications. This is supported by the significantly different protein and polysaccharide contents of the biofilms associated with rough and smooth surface types. Additionally, early phase biofilm genes (particularly icaA, icaC, and aap) are statistically significantly downregulated compared to the control at 48 h on rough surfaces. CoCrMo TiN and polished CoCrMo were the two smooth surface modifications which performed best on the basis of low biofilm content.