背景:脊髓是脊椎动物中枢神经系统的重要组成部分,控制运动以及接收和处理来自躯干和四肢的感觉信息。然而,有很多我们不知道这个重要的器官是如何发展的。这里,我们描述了21个转录因子和一个转录调节因子在斑马鱼脊髓中的表达。
结果:我们分析了aurkb的表达,foxb1a,foxb1b,her8a,homeza,ivns1abpb,mybl2b,myt1a,nr2f1b,onecut1,sall1a,sall3a,sall3b,sall4,sox2,sox19b,sp8b,tsc22d1,wdhd1,zfhx3b,znf804a,和野生型和MIBE3泛素蛋白连接酶1斑马鱼胚胎中的znf1032。虽然所有这些基因在脊髓中广泛表达,他们有不同的表达方式。一些主要在祖细胞结构域中表达,和其他有丝分裂后细胞亚群。鉴于脊髓发育的保守性,以及协调它的转录因子和转录调节因子,我们预计这些基因将在其他脊椎动物中具有相似的脊髓表达模式,包括哺乳动物和人类。
结论:我们的数据确定了22种不同的转录调节因子,它们是在脊髓发育中发挥不同作用的有力候选者。对于这些基因中的几个,这是对其脊髓表达的首次公开描述。
BACKGROUND: The spinal cord is a crucial part of the vertebrate CNS, controlling movements and receiving and processing sensory information from the trunk and limbs. However, there is much we do not know about how this essential organ develops. Here, we describe expression of 21 transcription factors and one transcriptional regulator in zebrafish spinal cord.
RESULTS: We analyzed the expression of aurkb, foxb1a, foxb1b, her8a, homeza, ivns1abpb, mybl2b, myt1a, nr2f1b, onecut1, sall1a, sall3a, sall3b, sall4, sox2, sox19b, sp8b, tsc22d1, wdhd1, zfhx3b, znf804a, and znf1032 in wild-type and MIB E3 ubiquitin protein ligase 1 zebrafish embryos. While all of these genes are broadly expressed in spinal cord, they have distinct expression patterns from one another. Some are predominantly expressed in
progenitor domains, and others in subsets of post-mitotic cells. Given the conservation of spinal cord development, and the transcription factors and transcriptional regulators that orchestrate it, we expect that these genes will have similar spinal cord expression patterns in other vertebrates, including mammals and humans.
CONCLUSIONS: Our data identify 22 different transcriptional regulators that are strong candidates for playing different roles in spinal cord development. For several of these genes, this is the first published description of their spinal cord expression.