短串联重复序列(STRs)和可变数量的串联重复序列(VNTRs)已在人类基因组中大约0.7和0.5万个基因座中被鉴定,分别,是高度多等位基因变异,而不是单核苷酸多态性。超过几千个STR的重复次数与附近基因的表达有关,这表明STR是影响人类性状的遗传变异。镇痛药通过其固有受体作用于中枢神经系统以产生镇痛作用。在本研究中,我们专注于CNR1、GRIN2A、PENK,和PDYN基因,并分析了术后疼痛管理中两个外周痛觉相关性状和七个镇痛相关性状。共有192名志愿者接受了外周疼痛感觉测试,139名和252名患者接受了开腹和正颌美容手术,分别,包括在研究中。四种STRs或VNTRs均不与周围疼痛有关。CNR1、GRIN2A、PENK基因与芬太尼的使用频率有关,芬太尼剂量,正颌整容手术后3小时疼痛评分和视觉模拟量表评分(分别为Spearman等级相关系数ρ=0.199,p=0.002,ρ=0.174,p=0.006和ρ=0.135,p=0.033),镇痛剂量,包括开腹手术后的硬膜外镇痛药(ρ=-0.200,p=0.018),正颌整容手术后24小时的视觉模拟疼痛评分(ρ=0.143,p=0.023),分别。CNR1基因中的STRs与正颌整容手术后芬太尼使用频率和芬太尼剂量之间的关联通过Holm的多重测试校正得到证实。这些发现表明,CNR1基因中的STR会影响口面区域的镇痛作用。
Short tandem repeats (STRs) and variable number of tandem repeats (VNTRs) that have been identified at approximately 0.7 and 0.5 million loci in the human genome, respectively, are highly multi-allelic variations rather than single-nucleotide polymorphisms. The number of repeats of more than a few thousand STRs was associated with the expression of nearby genes, indicating that STRs are influential genetic variations in human traits. Analgesics act on the central nervous system via their intrinsic receptors to produce analgesic effects. In the present study, we focused on STRs and VNTRs in the CNR1, GRIN2A, PENK, and PDYN genes and analyzed two peripheral pain sensation-related traits and seven analgesia-related traits in postoperative pain management. A total of 192 volunteers who underwent the peripheral pain sensation tests and 139 and 252 patients who underwent open abdominal and orthognathic cosmetic surgeries, respectively, were included in the study. None of the four STRs or VNTRs were associated with peripheral pain sensation. Short tandem repeats in the CNR1, GRIN2A, and PENK genes were associated with the frequency of fentanyl use, fentanyl dose, and visual analog scale pain scores 3 h after orthognathic cosmetic surgery (Spearman\'s rank correlation coefficient ρ = 0.199, p = 0.002, ρ = 0.174, p = 0.006, and ρ = 0.135, p = 0.033, respectively), analgesic dose, including epidural analgesics after open abdominal surgery (ρ = -0.200, p = 0.018), and visual analog scale pain scores 24 h after orthognathic cosmetic surgery (ρ = 0.143, p = 0.023), respectively. The associations between STRs in the CNR1 gene and the frequency of fentanyl use and fentanyl dose after orthognathic cosmetic surgery were confirmed by Holm\'s multiple-testing correction. These findings indicate that STRs in the CNR1 gene influence analgesia in the orofacial region.