背景:胆囊切除术是胆结石的成功治疗选择,尽管胆囊切除术(PC)后患者结直肠癌(CRC)的发病率显着增加。然而,目前尚不清楚升结肠粘膜微生物群的改变是否相关.
目的:探讨肠道菌群与胆囊切除术的潜在相关性。
方法:总共,30名PC患者和28名健康对照者接受结肠镜检查以收集粘膜活检样品。PC患者根据其临床特征进行分类。然后,16S-rRNA基因测序用于分析扩增子,阿尔法多样性,β多样性,和细菌群落的组成。此外,通过重建不受保护的国家对社区进行系统发育调查(PICRUSt)数据库,来自京都基因和基因组百科全书,用于预测细菌的功能。
结果:PC患者与健康对照组相当。然而,与60岁以下的PC患者相比,60岁以上的PC患者具有不同的组成。PC患者的门水平的拟杆菌丰富度要高得多。拟杆菌,副杆菌属,PC组比对照组更丰富。此外,PC患者表现出更丰富的代谢途径,特别是与脂多糖生物合成和万古霉素组抗生素生产有关的那些,而不是控制。
结论:这项研究表明,PC患者的粘膜菌群发生了改变,也许为胆囊切除术后CRC和腹泻的治疗可能性提供了新的观点。
BACKGROUND: Cholecystectomy is a successful treatment option for gallstones, although the incidence of colorectal cancer (CRC) has notably increased in post-cholecystectomy (PC) patients. However, it remains uncertain whether the altered mucosal microbiota in the ascending colon is related.
OBJECTIVE: To investigate the potential correlation between gut microbiota and the surgical procedure of cholecystectomy.
METHODS: In total, 30 PC patients and 28 healthy controls underwent colonoscopies to collect mucosal biopsy samples. PC patients were divided based on their clinical features. Then, 16S-rRNA gene sequencing was used to analyze the amplicon, alpha diversity, beta diversity, and composition of the bacterial communities. Additionally, the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) database, sourced from the Kyoto Encyclopedia of Genes and Genomes, was used to predict the functional capabilities of the bacteria.
RESULTS: PC patients were comparable with healthy controls. However, PC patients older than 60 years had a distinct composition compared to those under 60 years old. Bacteroidetes richness was considerably higher at the phylum level in PC patients. Bacteroides, Parabacteroides, and Bilophila were more abundant in the PC group than in the control group. Furthermore, PC patients exhibited greater enrichment in metabolic pathways, specifically those related to lipopolysaccharide biosynthesis and vancomycin group antibiotic production, than controls.
CONCLUSIONS: This study indicated that the mucosal microbiota in PC patients was altered, perhaps offering new perspectives on the treatment possibilities for CRC and diarrhea following cholecystectomy.