五环三萜类化合物(PCT),具有许多生物活性,被认为是次生植物代谢物的最重要类别之一。由于需要分离大量属于极性差异很大的几类结构相似的化合物(一元醇,二醇,和三萜酸)。这项研究提出了一种快速,敏感,和同时定量10个多氯三联苯的低成本方法(3β-taraxerol,lupeol,β-amyrin,α-amyrin,桦木醇,赤二醇,uvaol,桦木碱,齐墩果子,和熊果酸)通过高效液相色谱-串联质谱(HPLC-MS/MS)使用多孔石墨碳(Hypercarb)作为固定相,能够进行疏水保留和与分析物的特定相互作用。揭示流动相组成的影响,pH值,离子强度,在此基础上保留和选择色谱条件的柱温允许在8分钟内以梯度洗脱模式有效分离所有目标分析物,并在4-104µgL-1的范围内达到检测限。所开发的方法已得到充分验证,并成功地用于测定普通发帽(Polytrichumcommune)和草原泥炭藓(Sphagnumpalustre)苔藓中的PCT,和火草(Chamaenerionangustifolium)茎和叶。
Pentacyclic triterpenoids (PCTs), which possess a number of bioactive properties, are considered one of the most important classes of secondary plant metabolites. Their chromatographic determination in plant biomass is complicated by the need to separate a large number of structurally similar compounds belonging to several classes that differ greatly in polarity (monools, diols, and triterpenic acids). This study proposes a rapid, sensitive, and low-cost method for the simultaneous quantification of ten PCTs (3β-taraxerol, lupeol, β-amyrin, α-amyrin, betulin, erythrodiol, uvaol, betulinic, oleanolic, and ursolic acids) by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) using porous graphitic carbon (Hypercarb) as a stationary phase capable of hydrophobic retention and specific interactions with analytes. Revealing the effects of the mobile phase composition, pH, ionic strength, and column temperature on retention and selection of chromatographic conditions on this basis allowed for the effective separation of all target analytes within 8 min in gradient elution mode and attaining limits of detection in the range of 4-104 µg L-1. The developed method was fully validated and successfully tested in the determination of PCTs in common haircap (Polytrichum commune) and prairie sphagnum (Sphagnum palustre) mosses, and fireweed (Chamaenerion angustifolium) stems and leaves.