作为一种新型的人工酶,纳米酶是天然酶的理想替代品,已成功应用于许多领域。然而,在生物分子检测的应用中,大多数纳米酶具有反应时间长或检测限高的缺点,促使研究人员寻找新的高效纳米酶。在这项工作中,三种多金属氧酸盐基铁有机配合物([Fe(bpp)2](Mo6O19)的酶样活性,[Fe(bpp)2]2(Mo8O26)·2CH3OH,和[Fe(bpp)2]4H[Na(Mo8O26)]3),即,FeMo6、Fe2Mo8和Fe4Mo8Na,进行了分析。发现所有三种基于多金属氧酸盐的铁-有机络合物都能够催化过氧化氢(H2O2)氧化3,3',5,5'-四甲基联苯胺和邻苯二胺,导致可见的颜色变化,进一步表现出过氧化物酶样活性。结果表明,Fe4Mo8Na由于其长链结构而具有更多的活性位点,与Fe2Mo8和FeMo6相比,具有更突出的过氧化物酶样活性。树立了基于Fe4Mo8Na的H2O2和抗坏血酸检测比色传感平台。H2O2检测的线性响应范围为0.5-100μM,检测限为0.143μM。抗坏血酸检测的线性响应范围为0至750μM,检测极限为1.07μM。本研究为开发新的纳米酶和拓展纳米酶的传感和检测应用提供了新的视角。
As a new type of artificial enzyme, a nanozyme is an ideal substitute for natural enzymes and has been successfully applied in many fields. However, in the application of biomolecular detection, most nanozymes have the disadvantages of long reaction times or high detection limits, prompting researchers to search for new efficient nanozymes. In this work, the enzyme-like activities of three
polyoxometalate-based iron-organic complexes ([Fe(bpp)2](Mo6O19), [Fe(bpp)2]2(Mo8O26)·2CH3OH, and [Fe(bpp)2]4H[Na(Mo8O26)]3), namely, FeMo6, Fe2Mo8, and Fe4Mo8Na, were analyzed. All three
polyoxometalate-based iron-organic complexes were found to be capable of catalyzing hydrogen peroxide (H2O2) to oxidize 3,3\',5,5\'-tetramethylbenzidine and o-phenylenediamine, resulting in visible color changes, further exhibiting peroxidase-like activity. Results showed that Fe4Mo8Na had more active sites due to its long chain structure, endowing more prominent peroxidase-like activity compared with Fe2Mo8 and FeMo6. A colorimetric sensing platform for H2O2 and ascorbic acid detection based on Fe4Mo8Na was established. The linear response range for H2O2 detection was 0.5-100 μM, and the detection limit was 0.143 μM. The linear response for ascorbic acid detection ranges from 0 to 750 μM with a detection limit of 1.07 μM. This study provides a new perspective for developing new nanozymes and expanding the sensing and detection application of nanozymes.