几丁质-葡聚糖复合物(CGC)水凝胶是通过从碱性水溶液中凝结生物聚合物来制备的,和它们的形态,溶胀行为,机械,流变学,并对其生物学特性进行了研究。此外,评估了它们的体外药物负载/释放能力和通过模拟皮肤人工膜(Strat-M)的渗透。由4和6wt%的CGC悬浮液(Na51*4和Na51*6水凝胶,分别)聚合物含量为2.40±0.15和3.09±0.22wt%,分别,并显示出高度多孔的微观结构,其特征在于压缩模量为39.36和47.30kPa,储能模量为523.20和7012.25Pa,分别。两种水凝胶在水性介质中都具有自发且几乎立即的溶胀,和高保水能力(>80%),在37°C孵育30分钟后然而,Na51*4水凝胶具有较高的耐疲劳性和稍高的保水能力。这些水凝胶中含有咖啡因,布洛芬,双氯芬酸,或水杨酸,咖啡因的包封效率值达到13.11±0.49%,水杨酸为15.15±1.54%。对于所有测试的API,在PBS中观察到类似的释放曲线,包括最初的快速释放,然后是稳定的较慢的释放。使用Franz扩散池通过Strat-M膜进行的体外渗透实验显示,咖啡因(33.09µg/cm2/h)和水杨酸(19.53µg/cm2/h)的渗透通量高得多,与布洛芬钠和双氯芬酸钠(4.26和0.44µg/cm2/h,分别)。在正常人真皮成纤维细胞中的分析表明,CGC水凝胶对细胞的活力没有重大影响,迁移能力,和细胞的形态。鉴于他们展示的特点,CGC水凝胶是非常有前途的结构,显示可调物理属性,这支持了他们未来发展为新型透皮给药平台。
Chitin-glucan complex (CGC) hydrogels were fabricated by coagulation of the biopolymer from an aqueous alkaline solution, and their morphology, swelling behavior, mechanical, rheological, and biological properties were studied. In addition, their in vitro drug loading/release ability and permeation through mimic-skin artificial membranes (Strat-M) were assessed. The CGC hydrogels prepared from 4 and 6 wt% CGC suspensions (Na51*4 and Na51*6 hydrogels, respectively) had polymer contents of 2.40 ± 0.15 and 3.09 ± 0.22 wt%, respectively, and displayed a highly porous microstructure, characterized by compressive moduli of 39.36 and 47.30 kPa and storage moduli of 523.20 and 7012.25 Pa, respectively. Both hydrogels had a spontaneous and almost immediate swelling in aqueous media, and a high-water retention capacity (>80%), after 30 min incubation at 37 °C. Nevertheless, the Na51*4 hydrogels had higher fatigue resistance and slightly higher-water retention capacity. These hydrogels were loaded with caffeine, ibuprofen, diclofenac, or salicylic acid, reaching entrapment efficiency values ranging between 13.11 ± 0.49% for caffeine, and 15.15 ± 1.54% for salicylic acid. Similar release profiles in PBS were observed for all tested APIs, comprising an initial fast release followed by a steady slower release. In vitro permeation experiments through Strat-M membranes using Franz diffusion cells showed considerably higher permeation fluxes for caffeine (33.09 µg/cm2/h) and salicylic acid (19.53 µg/cm2/h), compared to ibuprofen sodium and diclofenac sodium (4.26 and 0.44 µg/cm2/h, respectively). Analysis in normal human dermal fibroblasts revealed that CGC hydrogels have no major effects on the viability, migration ability, and morphology of the cells. Given their demonstrated features, CGC hydrogels are very promising structures, displaying tunable physical properties, which support their future development into novel transdermal drug delivery platforms.