背景:由于环境中广泛存在丙烯酰胺,它可能是致癌物,以及猪模型作为人类类似物的适用性,作者决定评估高剂量和低剂量该化合物对猪骨髓红细胞生成过程的影响。
方法:在8周龄和体重约20kg的丹麦长白猪上进行实验。将动物分成三个相等的组,每组5只猪。对照动物接受空明胶胶囊(安慰剂)。来自第一实验组的动物接受0.5μg/kg体重/天的低剂量丙烯酰胺(纯度>99%;Sigma-Aldrich,波兰),第二个实验组的动物接受了10倍的剂量。安慰剂和丙烯酰胺胶囊每天早晨与饲料一起给药,持续28天。动物麻醉后,在第0天和第28天,将来自股骨的骨髓收集到不使用抗凝剂的试管中.干燥和染色后,使用光学显微镜对骨髓涂片进行详细的细胞学评估。
结果:这项研究表明,高剂量和低剂量的丙烯酰胺会影响猪的红细胞生成过程。丙烯酰胺对该过程的细胞毒性作用在多色成红细胞/正常成红细胞比率的变化中得到了证明。
结论:两种剂量的丙烯酰胺均导致正交和多色成红细胞数量的减少。
BACKGROUND: Due to the widespread occurrence of acrylamide in the environment, its likely carcinogen status, and the suitability of the pig model as a human analogue, the authors decided to evaluate the impact of high and low doses of this compound on the processes of erythropoiesis in swine bone marrow.
METHODS: The experiment was carried out on Danish Landrace pigs at the age of eight weeks and body weight about 20 kg. The animals were divided into three equal groups consisting of five pigs in each. Control animals received empty gelatin capsules (placebos). Animals from the first experimental group received a low dose of acrylamide of 0.5 μg/kg b.w./day (> 99% purity; Sigma-Aldrich, Poland), and animals from the second experimental group received a dose 10 times higher. Placebos and acrylamide capsules were administered with feed every morning for 28 days. After anaesthetisation of the animals, bone marrow from the femur was collected into tubes without an anticoagulant on days 0 and 28. After drying and staining, bone marrow smears were subjected to detailed cytological evaluation using a light microscope.
RESULTS: This study showed that high and low doses of acrylamide affected the process of porcine erythropoiesis. The cytotoxic effect of acrylamide on this process was demonstrated in a change of the polychromatic erythroblasts/normochromatic erythroblasts ratio.
CONCLUSIONS: Both doses of acrylamide caused a decrease in the number of ortho- and polychromatic erythroblasts.