The advent of metasurfaces has revolutionized the design of optical instruments, and recent advancements in fabrication techniques are further accelerating their practical applications. However, conventional top-down fabrication of intricate nanostructures proves to be expensive and time-consuming, posing challenges for large-scale production. Here, we propose a cost-effective bottom-up approach to create nanostructure arrays with arbitrarily complex meta-atoms displaying single nanoparticle lateral resolution over submillimeter areas, minimizing the need for advanced and high-cost nanofabrication equipment. By utilizing air/water interface assembly, we transfer nanoparticles onto templated polydimethylsiloxane (PDMS) irrespective of nanopattern density, shape, or size. We demonstrate the robust assembly of nanocubes into meta-atoms with diverse configurations generally unachievable by conventional methods, including U, L, cross, S, T, gammadion, split-ring resonators, and Pancharatnam-Berry metasurfaces with designer optical functionalities. We also show nanocube epitaxy at near ambient temperature to transform the meta-atoms into complex continuous nanostructures that can be swiftly transferred from PDMS to various substrates via contact printing. Our approach potentially offers a large-scale manufacturing alternative to top-down fabrication for metal nanostructuring, unlocking possibilities in the realm of nanophotonics.

The vibrational assignment of the Raman and surface-enhanced Raman scattering (SERS) spectra of the herbicide tebuthiuron (TBH) was accomplished, which allowed unprecedented propositions for adsorption geometries on the surface of silver nanoparticles (AgNP). Ascribed SERS features allowed suggesting that the adsorption occurred through nitrogen atoms of thiadiazole group, since intense band shift assigned to ring mode was marking of the coordination with the metallic surface. AgNP were treated with different surface modifiers that leaded to substantial changes in TBH adsorption geometries. Spectral changes, as the enhancement of out-of-plane ring modes, were indicative of the presence of tilted thiadiazole geometries in relation to the silver surface. Density Functional Theory (DFT) calculations from TBH molecules, in isolation and in interaction with ten-atom cluster of silver leaded to obtain theoretical spectra that gave support to interpret experimental Raman and SERS spectra.

Green energy technology is generally becoming one of hot issues that need to be solved due to the adverse effects on the environment of fossil fuels. One of the strategies being studied and developed by theorists and experimentalists is the use of photoelectrochemical (PEC) cells, which are emerging as a candidate to produce hydrogen from water splitting. However, creating photoelectrodes that meet the requirements for PEC water splitting has emerged as the primary obstacle in bringing this technology to commercial fruition. Here, we construct a heterostructure, which consists of MoS2/TiO2/Au nanoparticles (NPs) to overcome the drawbacks of the photoanode. Owing to the dependence on charge transfer, the bandgap of MoS2/TiO2and the utilization the Au NPs as a stimulant for charges separation of TiO2by localized surface plasmon resonances effect as well as the increase of hot electron injection to cathode, leading to photocatalytic activities are improved. The results have recorded a significant increase in the photocurrent density from 2.3μAcm-2of TiO2to approximately 16.3μAcm-2of MoS2/TiO2/Au NPs. This work unveils a promising route to enhance the visible light adsorption and charge transfer in photo-electrode of the PEC cells by combining two-dimensional materials with metal NPs.

The limitations of conventional template-based methods for the deposition of nanoparticle assemblies into defined patterns on solid substrates call for the development of techniques that do not require templates or lithographic masks. The use of optically-induced thermal gradients to drive the migration of colloids toward or away from a laser spot, known as opto-thermophoresis, has shown promise for the low-power trapping and optical manipulation of a variety of colloidal species. However, the printing of colloids using this technique has so far not been established. Herein, a method for the optically directed printing of noble metal nanoparticles, specifically gold nanospheres is reported. The thermophoresis of the polymer polyvinylpyrrolidone and gold nanospheres toward a laser spot led to the deposition of nanoparticle aggregates, capable of serving as surface-enhanced Raman scattering substrates. The influence of heating laser power and the concentrations of polymer, salt, and surfactant on the nanoparticle deposition rate and structure of the printed pattern are studied, showing that a variety of conditions can permit printing, suggesting facile generalization to different nanoparticle compositions, sizes, and shapes. These findings will greatly benefit future efforts for directed nanoparticle assembly, and drive applications in sensing, photothermal heating, and relevant applications in biomedicine and devices.

Surface-engineered gold nanoparticles have been considered as versatile systems for theranostics applications. Moreover, surface covering or stabilizing agents on gold nanoparticles especially gold nanobipyramids (AuNBPs) provides an extra space for cargo molecules entrapment. However, it is not well studied yet and also the preparation of AuNBPs still remains dependent largely on cetyltrimethylammonium bromide (CTAB), a cytotoxic surfactant. Therefore, the direct use of CTAB stabilized nanoparticles is not recommended for cancer theranostics applications. Herein, we address an approach of dodecyl ethyl dimethylammonium bromide (DMAB) as biocompatible structure directing agent for AuNBPs, which also accommodate anticancer drug doxorubicin (45%), an additional chemotherapeutics agent. Upon near-infrared light (NIR, 808 nm) exposure, engineered AuNBPs exhibit (i) better phototransduction (51 °C) due to NIR absorption ability (650-900 nm), (ii) photo triggered drug release (more than 80%), and (iii) synergistic chemophototherapy for breast cancer cells. Drug release response has been evaluated in tumor microenvironment conditions (84% in acidic pH and 80% at high GSH) due to protonation and high affinity of thiol binding with AuNBPs followed by DMAB replacement. Intracellular glutathione (GSH, 5-7.5 mM) replaces DMAB from AuNBPs, which cause easy aggregation of nanoparticles as corroborated by colorimetric shifts, suggesting their utilization as a molecular sensing probe of early stage cancer biomarkers. Our optimized recipe yield is monodisperse DMAB-AuNBPs with ∼90% purity even at large scales (500 mL volume per batch). DMAB-AuNBPs show better cell viability (more than 90%) across all concentrations (5-500 ug/mL) when directly compared to CTAB-AuNBPs (less than 10%). Our findings show the potential of DMAB-AuNBPs for early stage cancer detection and theranostics applications.

Throughout the central nervous system, the spinal cord plays a very important role, namely, transmitting sensory and motor information inwardly so that it can be processed by the brain. There are many different ways this structure can be damaged, such as through traumatic injury or surgery, such as scoliosis correction, for instance. Consequently, damage may be caused to the nervous system as a result of this. There is no doubt that optical devices such as microscopes and cameras can have a significant impact on research, diagnosis, and treatment planning for patients with spinal cord injuries (SCIs). Additionally, these technologies contribute a great deal to our understanding of these injuries, and they are also essential in enhancing the quality of life of individuals with spinal cord injuries. Through increasingly powerful, accurate, and minimally invasive technologies that have been developed over the last decade or so, several new optical devices have been introduced that are capable of improving the accuracy of SCI diagnosis and treatment and promoting a better quality of life after surgery. We aim in this paper to present a timely overview of the various research fields that have been conducted on optical devices that can be used to diagnose spinal cord injuries as well as to manage the associated health complications that affected individuals may experience.

Numerous experiments have demonstrated improvements on the efficiency of perovskite solar cells by introducing plasmonic nanoparticles, however, the underlying mechanisms are still not clear: the particles may enhance light absorption and scattering, as well as charge separation and transfer, or the perovskite\'s crystalline quality. Eventually, it can still be debated whether unambiguous plasmonic increase of light absorption has indeed been achieved. Here, various optical models are employed to provide a physical understanding of the relevant parameters in plasmonic perovskite cells and the conditions under which light absorption may be enhanced by plasmonic mechanisms. By applying the recent generalized Mie theory to gold nanospheres in perovskite, it is shown that their plasmon resonance is conveniently located in the 650-800 nm wavelength range, where absorption enhancement is most needed. It is evaluated for which active layer thickness and nanoparticle concentration a significant enhancement can be expected. Finally, the experimental literature on plasmonic perovskite solar cells is analyzed in light of this theoretical description. It is estimated that only a tiny portion of these reports can be associated with light absorption and point out the importance of reporting the perovskite thickness and nanoparticle concentration in order to assess the presence of plasmonic effects.

As a single-particle characterization technique, optical microscopy has transformed our understanding of structure-function relationships of plasmonic nanoparticles, but the need for ex-situ-correlated electron microscopy to obtain structural information handicaps an otherwise exceptional high-throughput technique. Here, we present an all-optical alternative to electron microscopy to accurately and quickly extract structural information about single gold nanorods (Au NRs) using calcite-assisted localization and kinetics (CLocK) microscopy. Color CLocK images of single Au NRs allow scattering from the longitudinal and transverse plasmon modes to be imaged simultaneously, encoding spectral data in CLocK images that can then be extracted to obtain Au NR size and orientation. Moreover, through the use of convolutional neural networks, Au NR length, width, and aspect ratio can be predicted directly from color CLocK images within ∼10% of the true value measured by electron microscopy.

BACKGROUND: C-reactive protein (CRP) represents an early clinical biomarker that indicates the presence of inflammatory or infectious conditions in the human body. Today\'s procedures approved by the Food and Drug Administration (FDA) imply expensive equipment and highly trained personnel to perform the test. Therefore, a new diagnostic method with high detection efficiency and less cost is urgently needed for delivering rapid and timely results in point-of-care (POC) service.
RESULTS: Herein, we propose a new, equipment-free, and portable sensing method for the future POC detection of CRP based on the Tyndall effect (TE). In our study, aptamer-conjugated citrate-stabilized gold nanoparticles (apta-AuNPs) are exploited as the sensing platform. The apta-AuNPs\' interaction with CRP in a saline environment leads to their aggregation, thus enhancing the scattering of light when the solution is exposed to a 640 nm pointer laser line. Firstly, the enhancement of the scattering light as a function of increasing concentration of CRP in solution is measured spectroscopically using a typical 90-degree angle spectrofluorometer and then the measurements are compared to the classic colorimetric detection using an UV-Vis spectrophotometer. Finally, to achieve high portability and accessibility, we demonstrate that the measurement of CRP concentration can be performed with similar accuracy but in a more direct and inexpensive way by using a laser pointer pen as the excitation source and a camera of a low-budget smartphone as a quantitative reader instead of most expensive spectrofluorometer.
CONCLUSIONS: The portable TE-based assay exhibits a wide linear dynamic range (1-60 μg/mL) for the detection of CRP with a limit of detection (LOD) of 92 ng/mL The proposed method is capable to integrate both standard and high-sensitivity CRP analysis in a single procedure with increased sensitivity and prompt delivery of analysis results. Moreover, the sensing procedure is significantly faster than the FDA approved ones with a detection time of only 10 min. Finally, as a proof-of-concept, our findings demonstrate excellent recovery for CRP detection in spiked and diluted urine samples, highlighting the strong potential of this sensing method for POC applications.

Fungal infections are a significant global health problem, particularly affecting individuals with weakened immune systems. Moreover, as uncontrolled antibiotic and immunosuppressant use increases continuously, fungal infections have seen a dramatic increase, with some strains developing antibiotic resistance. Traditional approaches to identifying fungal strains often rely on morphological characteristics, thus owning limitations, such as struggles in identifying several strains or distinguishing between fungal strains with similar morphologies. This review explores the multifaceted impact of fungi infections on individuals, healthcare providers, and society, highlighting the often-underestimated economic burden and healthcare implications of these infections. In light of the serious constraints of traditional fungal identification methods, this review discusses the potential of plasmonic nanoparticle-based biosensors for fungal infection identification. These biosensors can enable rapid and precise fungal pathogen detection by exploiting several readout approaches, including various spectroscopic techniques, colorimetric and electrochemical assays, as well as lateral-flow immunoassay methods. Moreover, we report the remarkable impact of plasmonic Lab on a Chip technology and microfluidic devices, as they recently emerged as a class of advanced biosensors. Finally, we provide an overview of smartphone-based Point-of-Care devices and the associated technologies developed for detecting and identifying fungal pathogens.