■辣木和紫菜被广泛用作食品的成分,并在传统药物中用于治疗各种疾病。
■对辣木叶和山毛虫茎的提取物进行了评估,以检查它们抑制氧化性DNA损伤的能力(通过DNA保护试验),在V79细胞中单独和组合的细胞保护和基因保护潜能(通过彗星测定)。
■发现这些提取物可以显着抑制pUC18质粒DNA的OH依赖性损伤。油乳杆菌提取物(160和320μg/mL)和紫杉孢菌提取物(640、1,280和2,560μg/mL)分别显示出较高的DNA保护活性。在V79细胞系中,在不同浓度的组合和不同浓度的单个植物中,油菌(1,280μg/mL)与Tinosporacordifolia(640μg/mL)的组合对过氧化氢诱导的细胞毒性和遗传毒性表现出最佳的细胞保护和基因保护活性。
■本研究证明了油菌和丝缕孢菌单独或组合的细胞保护和基因保护活性。
UNASSIGNED: Moringa oleifera and Tinospora cordifolia is extensively used as an ingredient of food and in traditional medicine for the management of a variety of diseases.
UNASSIGNED: The extracts of leaf of Moringa oleifera and stem of Tinospora cordifolia were assessed to examine their ability to inhibit the oxidative DNA damage (by DNA protection assay), cytoprotective and genoprotective potential (by Comet assay) in V79 cells individually and in combinations.
UNASSIGNED: It was found that these extracts could significantly inhibit the OH-dependent damage of pUC18 plasmid DNA. M. oleifera extract (160 and 320 μg/mL) and Tinospora cordifolia extract (640, 1,280 and 2,560 μg/mL) individually showed higher DNA protection activity. M. oleifera (1,280 μg/mL) combined with Tinospora cordifolia (640 μg/mL) showed best cytoprotective and genoprotective activities among different concentration combinations and various concentrations of individual plants in V79 cell line against hydrogen peroxide induced cytotoxicity and genotoxicity.
UNASSIGNED: This study demonstrates the cytoprotective and genoprotective activity of M. oleifera and Tinospora cordifolia individually or in combination.